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Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform

  • Metabolic Engineering and Synthetic Biology - Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Yarrowia lipolytica is an oleaginous yeast that has been substantially engineered for production of oleochemicals and drop-in transportation fuels. The unique acetyl-CoA/malonyl-CoA supply mode along with the versatile carbon-utilization pathways makes this yeast a superior host to upgrade low-value carbons into high-value secondary metabolites and fatty acid-based chemicals. The expanded synthetic biology toolkits enabled us to explore a large portfolio of specialized metabolism beyond fatty acids and lipid-based chemicals. In this review, we will summarize the recent advances in genetic, omics, and computational tool development that enables us to streamline the genetic or genomic modification for Y. lipolytica. We will also summarize various metabolic engineering strategies to harness the endogenous acetyl-CoA/malonyl-CoA/HMG-CoA pathway for production of complex oleochemicals, polyols, terpenes, polyketides, and commodity chemicals. We envision that Y. lipolytica will be an excellent microbial chassis to expand nature’s biosynthetic capacity to produce plant secondary metabolites, industrially relevant oleochemicals, agrochemicals, commodity, and specialty chemicals and empower us to build a sustainable biorefinery platform that contributes to the prosperity of a bio-based economy in the future.

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Acknowledgements

This work was supported by the Bill and Melinda Gates Foundation (Grant number OPP1188443) and the Cellular and Biochem Engineering Program of the National Science Foundation under Grant no.1805139.

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Authors and Affiliations

  1. Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA

    Jingbo Ma, Yang Gu, Monireh Marsafari & Peng Xu

  2. Department of Agronomy and Plant Breeding, University of Guilan, Rasht, Islamic Republic of Iran

    Monireh Marsafari

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  1. Jingbo Ma
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  2. Yang Gu
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  3. Monireh Marsafari
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  4. Peng Xu
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Correspondence to Peng Xu.

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Ma, J., Gu, Y., Marsafari, M. et al. Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform. J Ind Microbiol Biotechnol 47, 845–862 (2020). https://doi.org/10.1007/s10295-020-02290-8

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