Abstract
The oomycete Phytophthora melonis causes a severe disease in cucumber plants in Asia. In this study, the diameter of cucumber hypocotyl in the resistant variety ‘Shantou qing gua’ was significantly larger than that of the susceptible variety ‘Zhongnong No. 20’. The significantly lower incidence of disease and less invasive hyphae on the epidermis and transverse section of hypocotyls in P plants of the resistant variety than those in susceptible cultivars were also observed. Brassinosteroids are a class of phytohormones that affect plant growth and development and are involved in regulating plant resistance to a variety of biotic and abiotic stresses. 24-Epibrassinolide root drenching significantly enhanced the thickening of cucumber hypocotyl. Thick hypocotyls showed strong resistance to P. melonis, indicating that it significantly reduced the incidence of disease and retarded the hyphae extension for both resistant and susceptible cucumbers. 24-Epibrassinolide pretreatment had no significant effect on the elongation of cucumber hypocotyl. Further histological observation showed that under the condition of infection with P. melonis, exogenous 24-epibrassinolide could induce lignin deposition in external phloem and xylem vessel cell wall of the cucumber hypocotyl vascular bundle. There is also an accumulation of callose in the external phloem sieve plate, which activates the resistance responses in cell walls. It is worth mentioning that in both inoculated and uninoculated conditions, exogenous 24-epibrassinolide enhanced lignin formation in external phloem and xylem vessel cell wall of the vascular bundle. This increased the content of lignin in hypocotyl as well as the number of vascular bundles in the hypocotyl base. The above results show that 24-epibrassinolide constitutively regulates the thickening of cucumber hypocotyl and the development of vascular bundle, hence preventing phytophthora infection and inducing plant resistance to disease.
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Acknowledgments
This study was funded by the National Natural Science Foundation of China (31872090, 31301767), and the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (No. 2019KJ122).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Rongrong Ren, Aiting Song, Chenchen Li, HaijunYang, and Xian Yang. The first draft of the manuscript was written by Yunyan Kang, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ren, R., Yang, X., Song, A. et al. Control of Phytophthora melonis damping-off treated with 24-epibrassinolide and a histological study of cucumber hypocotyl. Protoplasma 257, 1519–1529 (2020). https://doi.org/10.1007/s00709-020-01523-y
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DOI: https://doi.org/10.1007/s00709-020-01523-y