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Effects of Selenium on Serotonin Synthesis and the Glutathione Redox Cycle in Plum Leaves

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Abstract

Selenium (Se), an essential micronutrient for humans, is also a beneficial nutrient for growth and development of many plants. The aim of the present study was to determine whether low-dose Se application could increase plum growth and serotonin synthesis, as well as upregulate the glutathione redox cycle. Two-year-old plum trees were treated with Se6+ (Na2SeO4) and Se2− (SeMet). Growth parameters, levels of serotonin synthesis precursors, compounds and enzymes of the glutathione (GSH) redox cycle, and the antioxidant capacity were analyzed. Results showed that plant height and stem diameter were increased by Se treatment. The leaf Se content was significantly increased; tryptophan was not affected by Se treatment, while the 5-hydroxytryptophan content was significantly higher than control. Although serotonin levels were also increased, the effect was not significant, and no tryptamine was detected in our study. On the 30th day, the MDA content in Se2− was highest followed by the control and Se6+ treatment, and the activities of T-AOT and CAT were increased, while GST was reduced by Se treatment. The two forms of Se treatments increased the activity of GPX and GR, and the total content of GSH + glutathione disulfide (GSSG) at the end of treatment. Furthermore, the level of GSH and GSH/GSSG was instable changed. GSSG content was similar during the treatment time except on the 20 days. Conclusively, the pathway of plum serotonin was clear, both Se6+ and Se2− could influence the serotonin synthesis. Additionally, the GSH redox cycle was sensitive to the Se application. Moreover, Se6+ performed better than Se2− on leaf Se content, plant growth parameters, and antioxidant capacity.

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References

  • Balakhnina TI, Nadezhkina ES (2017) Effect of selenium on growth and antioxidant capacity of Triticum aestivum L. during development of lead-induced oxidative stress. Russ J Plant Physl 64:215–223

    CAS  Google Scholar 

  • Backlund PS, Urbanski HF, Doll MA, Hein DW, Bozinoski M, Mason CE, Coon SL, Klein DC (2017) Daily rhythm in plasma N-acetyltryptamine. J Biol Rhythm 32:195–211

    CAS  Google Scholar 

  • Banuelos GS, Arroyo I, Pickering IJ, Yang SI, Freeman JL (2015) Selenium biofortification of broccoli and carrots grown in soil amended with Se–enriched hyperaccumulator Stanleya pinnata. Food Chem 166:603–608

    CAS  PubMed  Google Scholar 

  • Bela K, Horvath E, Galle A, Szabados L, Tari I, Csiszar J (2015) Plant glutathione peroxidases: emerging role of the antioxidant enzymes in plant development and stress responses. J Plant Physiol 176:192–201

    CAS  PubMed  Google Scholar 

  • Carmagnol F, Sinet PM, Jerome H (1983) Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts. BBA-Gen Subject 759:49–57

    CAS  Google Scholar 

  • Cartes P, Gianfreda L, Mora ML (2005) Uptake of selenium and its antioxidant activity in ryegrass when applied as selenate and selenite forms. Plant Soil 276:359–367

    CAS  Google Scholar 

  • Cartes P, Gianfreda L, Paredes C, Mora ML (2011) Selenium uptake and its antioxidant role in ryegrass cultivars as affected by selenite seed palletization. J Soil Sci Plant Nutr 11:1–14

    Google Scholar 

  • Delgado J, Terron MP, Garrido M, Pariente JA, Barriga C, Rodríguez AB, Paredes SD (2012) A cherry nutraceutical modulates melatonin, serotonin, corticosterone, and total antioxidant capacity levels: effect on ageing and chronotype. J Appl Biomed 10:109–117

    CAS  Google Scholar 

  • Fellows LE, Bell EA (1970) 5-Hydroxy-L-tryptophan, 5-hydroxytryptamine and L-tryptophan-5-hydroxylase in Griffonia simplicifolia. Phytochemistry 9:2389–2396

    CAS  Google Scholar 

  • Ferreira RLDC, Prado RDM, Junior JPDS, Gratão PL, Tezotto T, Cruz FJR (2020) Oxidative stress, nutritional disorders, and gas exchange in lettuce plants subjected to two selenium sources. J Soil Sci Plant Nutr. https://doi.org/10.1007/s42729-020-00206-0

  • Foyer CH, Lopez DH, Dat JF, Scott IM (1997) Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiol Plant 100:241–254

    CAS  Google Scholar 

  • Galle A, Czekus Z, Bela K, Horvath E, Ordog A, Csiszar J, Poor P (2019) Plant glutathione transferases and light. Front Plant Sci 9

  • Garrido M, Gonzalez-Gomez D, Lozano M, Barriga C, Rodriguez AB (2013) A Jerte valley cherry product provides beneficial effects on sleep quality, influence on aging. J Nutr Health Aging 17:553–560

    CAS  PubMed  Google Scholar 

  • Grant K, Carey N, Grant K, Carey N, Mendoza M, Schulze J, Pilon M, Pilon-Smits EAH, Pilon-Smit HDV (2011) Adenosine 5′-phosphosulfate reductase (APR2) mutation in Arabidopsis implicates glutathione deficiency in selenate toxicity. Biochem J 438:325–335

    CAS  PubMed  Google Scholar 

  • Gonzalez-Flores D, Velardo B, Garrido M, Gonzalez-Gomez D, Lozano M, Ayuso MC, Barriga C, Paredes SD, Rodriguez AB (2011) Ingestion of Japanese plums (Prunus salicina Lindl. cv. Crimson Globe) increases the urinary 6-sulfatoxymelatonin and total antioxidant capacity levels in young, middle-aged and elderly humans: nutritional and functional characterization of their content. J Food and Nutr Res 50:229–236

    CAS  Google Scholar 

  • Guerrero B, Llugany M, Palacios O, Valiente M (2014) Dual effects of different selenium species on wheat. Plant Physiol Biochem 83:300–307

    CAS  PubMed  Google Scholar 

  • Hou X, Tan L, Tang SF (2019) Molecular mechanism study on the interactions of cadmium (II) ions with Arabidopsis thaliana glutathione transferase Phi8. Spectrochim ACTAA 216:411–471

    CAS  Google Scholar 

  • Huang X, Mazza G (2011) Simultaneous analysis of serotonin, melatonin, piceid and resveratrol in fruits using liquid chromatography tandem mass spectrometry. J Chromatogr A 1218:3890–3899

    CAS  PubMed  Google Scholar 

  • Islam J, Shirakawa H, Nguyen TK, Aso H, Komai M (2016) Simultaneous analysis of serotonin, tryptophan and tryptamine levels in common fresh fruits and vegetables in Japan using fluorescence HPLC. Food Biosci 13:56–59

    CAS  Google Scholar 

  • Jiang C, Zu C, Shen J, Shao F, Li T (2015) Effects of selenium on the growth and photosynthetic characteristics of flue-cured tobacco (Nicotiana tabacum L.). Acta Soc Bot Pol 84:71–77

    CAS  Google Scholar 

  • Kapoor R, Singh N (2017) Arbuscular mycorrhiza and reactive oxygen species. In: Wu QS (Ed.), arbuscular mycorrhizas and stress tolerance of plants. Springer Nature Singapore Pte Ltd., Singapore: 25–42

  • Kikkert J, Berkelaar E (2013) Plant uptake and translocation of inorganic and organic forms of selenium. Arch Environ Contam Toxicol 65:458–465

    CAS  PubMed  Google Scholar 

  • Kumar S, Asif MH, Chakrabarty D, Tripathi RD, Trivedi PK (2013) Differential expression of rice lambda class GST gene family members during plant growth, development, and in response to stress conditions. Plant Mol Biol Report 31:569–580

    CAS  Google Scholar 

  • Lei XG, Evenson JK, Thompson KM, Sunde RA (1995) Glutathione peroxidases and phospholipids hydro peroxide glutathione peroxidases are differentially regulated in rats by dietary selenium. J Nutr 125:1438–1446

    CAS  PubMed  Google Scholar 

  • Li MQ, Hasan MK, Li CX, Ahammed GJ, Xia XJ, Shi K, Zhou YH, Reiter RJ, Yu JQ, Xu MX (2016) Melatonin mediates selenium-induced tolerance to cadmium stress in tomato plants. J Pineal Res 61:291–302

    CAS  PubMed  Google Scholar 

  • Ly D, Kang K, Choi JY, Ishihara BK, Lee SG (2008) HPLC analysis of serotonin, tryptamine, tyramine, and the hydroxycinnamic acid amides of serotonin and tryamine in food vegetables. J Med Food 11:385–389

    CAS  PubMed  Google Scholar 

  • Malheiros RSP, Gonçalves FCM, Brito FAL, Zsogon A, Ribeiro DM (2020) Selenomethionine induces oxidative stress and modifies growth in rice (Oryza sativa L.) seedlings through effects on hormone biosynthesis and primary metabolism. Ecotoxicol Environ 189:109942

  • Martin AM, Young RL, Leong L, Rogers GB, Spencer NJ, Jessup CF, Keating DJ (2017) The diverse metabolic roles of peripheral serotonin. Endocrinology 158:1049–1063

    CAS  PubMed  Google Scholar 

  • Martina P, Fernando M, Pezzarossa B (2017) Selenium enrichment of horticultural crops. Molecules 22:933

    Google Scholar 

  • Metzner L, Kottra G, Neubert K, Daniel H, Brandsch M (2005) Serotonin, L-tryptophan, and tryptamine are effective inhibitors of the amino acid transport system PAT1. FASEB J 19:1468–1473

    CAS  PubMed  Google Scholar 

  • Naeem K, Jeong IJ, Hwang IM, Kim JS, Choi SH, Nho EY, Choi JY, Kwak BM, Ahn JH, Yoon T, Kim KS (2013) Method validation for simultaneous determination of chromium, molybdenum and selenium in infant formulas by icp-oes and icp-ms. Food Chem 141:3566–3570

    Google Scholar 

  • Ogra Y, Katayama A, Ogihara Y, Yawata A, Anan Y (2013) Analysis of animal and plant selenometabolites in roots of a selenium accumulator, brassica rapa var. peruviridis, by speciation. Metallomics 5:429

    CAS  PubMed  Google Scholar 

  • Ozen IT, Ekşi A (2016) Melatonin and serotonin content of the main sour cherry varieties and commercially produced sour cheery concentrates. Eur Int J Sci Technol 5:57–64

    Google Scholar 

  • Park M, Kang K, Park S, Back K (2008) Conversion of 5-hydroxytryptophan into serotonin by tryptophan decarboxylase in plants, Escherichia coli, and yeast. Biosci Biotechnol Biochem 72:2456–2458

    CAS  PubMed  Google Scholar 

  • Park S, Lee K, Kim YS, Kyoungwhan B (2012) Tryptamine 5-hydroxylase-deficient Sekiguchi rice induces synthesis of 5-hydroxytryptophan and N-acetyltryptamine but decreases melatonin biosynthesis during senescence process of detached leaves. J Pineal Res 52:211–216

    CAS  PubMed  Google Scholar 

  • Perez M, Lipinski V, Filippini M, Chacon-Madrid K, Arruda M, Wuilloud R (2019) Selenium biofortification on garlic growth and other nutrients accumulation. Hortic Bras 37:294–301

    Google Scholar 

  • Posmyk MM, Kontek R, Janas KM (2009) Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress. Ecotox Environ Safe 72:596–602

    CAS  Google Scholar 

  • Saleem MF, Kamal MA, Shahid M, Saleem A, Shakeel A, Anjum SA (2020) Exogenous selenium-instigated physiochemical transformations impart terminal heat tolerance in Bt cotton. J Soil Sci Plant Nutr 20:274–283

    CAS  Google Scholar 

  • Seppanen M, Turakainen M, Hartikainen H (2003) Selenium effects on oxidative stress in potato. Plant Sci 165:311–319

    CAS  Google Scholar 

  • Shahnazari M, Turner RT, Iwaniec UT, Wronski TJ, Li M, Ferruzzi MG, Nissenson RA, Halloran BP (2016) Dietary dried plum increases bone mass, suppresses proinflammatory cytokines and promotes attainment of peak bone mass in male mice. J Nutr Biochem 34:73–82

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shan C, Wang B, Sun H, Gao S, Li H (2020) H2S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress. Protoplasma. https://doi.org/10.1007/s00709-020-01510-3

  • Chai SC, Hooshmand S, Saadat RL, Payton ME, Brummel-Smith K, Arjmandi BH (2012) Daily apple versus dried plum: impact on cardiovascular disease risk factors in postmenopausal women. J Acad Nutr Diet 112:1158–1168

    PubMed  Google Scholar 

  • Sors TG, Ellis DR, Salt DE (2005) Selenium uptake, translocation, assimilation and metabolic fate in plants. Photosynth Res 86:373–389

    CAS  PubMed  Google Scholar 

  • Stolfa I, Velki M, Vukovic R, Ecimovic S, Katanic Z, Loncaric Z (2017) Effect of different forms of selenium on the plant-soil-earthworm system. J Plant Nutr Soil Sci 180:231–240

    CAS  Google Scholar 

  • Sun X, Yi H, Chen Y, Luo Y, Tan P, Xie Y (2018) Effects of different concentrations of Se6+ on selenium absorption, transportation, and distribution of citrus seedlings (C. junos cv. Ziyang xiangcheng). J Plant Nutr 2:168–177

    Google Scholar 

  • Sun X, Wang Y, Han G, Ye S, Zhou X (2020) Effects of different selenium forms on selenium accumulation, plant growth, and physiological parameters of wild peach. South Afri J Bot 131:437–442

    CAS  Google Scholar 

  • Takeda T (2015) Post-translational activation of non-selenium glutathione peroxidase of Chlamydomonas reinhardtii by specific incorporation of selenium. Biochem Biophys Rep 4:39–43

    PubMed  PubMed Central  Google Scholar 

  • Tan DX, Manchester LC, Esteban-Zubero E, Zhou Z, Reiter JR (2015) Melatonin as a potent and inducible endogenous antioxidant: synthesis and metabolism. Molecules 20:18886–18906

    CAS  PubMed  PubMed Central  Google Scholar 

  • Terry N, Zayed AM, De Souza MP, Tarum AS (2000) Selenium in higher plants. Annu Rev Plant Physiol Plant Mol Biol 51:401–432

    CAS  PubMed  Google Scholar 

  • Thangavel K (2017) Selenium properties for anti-cancer. Res J Pharm Technol 10:3595–3597

    Google Scholar 

  • Wang CQ, Xu HJ, Liu T (2011) Effect of selenium on ascorbate–glutathione metabolism during PEG-induced water deficit in Trifolium repens L. J Plant Growth Regul 30:436–444

    CAS  Google Scholar 

  • Wang M, Dinh QT, Qi M, Wang M, Yang W, Zhou F, Liang D (2019) Radicular and foliar uptake, and xylem- and phloem-mediated transport of selenium in maize (Zea mays L.): a comparison of five Se exogenous species. Plant Soil 6:1–13

    Google Scholar 

  • Wu ZC, Liu S, Zhao J, Wang FH, Du YQ, Zou SM, Li HM, Wen D, Huang YD (2017) Comparative responses to silicon and selenium in relation to antioxidant enzyme system and the glutathione-ascorbate cycle in flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis) under cadmium stress. Environ Exp Bot 133:1–11

    Google Scholar 

  • Xue T, Hartikainen H, Piironen V (2001) Antioxidative and growth promoting effect of selenium in senescing lettuce. Plant Soil 237:55–61

    CAS  Google Scholar 

  • Yao S, Chen S, Xu D, Lan H (2010) Plant growth and responses of antioxidants of Chenopodium album to long-term NaCl and KCl stress. Plant Growth Regul 60:115–125

    CAS  Google Scholar 

  • Zhao Y, Tan DX, Lei Q, Chen H, Wang L, Li Q, Gao Y, Kong J (2013) Melatonin and its potential biological functions in the fruits of sweet cherry. J Pineal Res 55:79–88

    CAS  PubMed  Google Scholar 

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Funding

This study was funded by the Basic Research and Frontier Exploration Project of Chongqing Municipality (grant number cstc2018jcyjAX0678) and the National Natural Science Foundation of China (grant number 31901972).

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Authors and Affiliations

  1. School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, 408100, China

    Xieping Sun, Guoqiang Han, Shuang Ye & Xianrong Zhou

  2. Fruit Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing, 401329, China

    Youjin Luo

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  1. Xieping Sun
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Sun, X., Han, G., Ye, S. et al. Effects of Selenium on Serotonin Synthesis and the Glutathione Redox Cycle in Plum Leaves. J Soil Sci Plant Nutr 20, 2212–2221 (2020). https://doi.org/10.1007/s42729-020-00288-w

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