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Impact of Initial Feeding and Molting on Tachypleus tridentatus Gut Microbiota

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Abstract

Tri-spine horseshoe crabs (HSCs) Tachypleus tridentatus have been facing the threat of population depletion for decades, and the physiology and microbiology of their early life stages are lacking. To explore what directs the change of juvenile T. tridentatus gut microbiota and how gut microbiota change, by using 16S rRNA sequencing of gut samples we detected the intestinal microbiome of juvenile HSCs and compared the impact of initial molting and initial feeding, as well as the effect of environment. Results showed that the predominant phyla in the gut microbial community of juvenile HSCs are Proteobacteria and Bacteroidetes. The richness and diversity of intestinal microbes greatly decreased after initial molting. Microbial-mediated functions predicted by PICRUSt showed that “Signal Transduction”, “Cellular Processes and Signaling”, “Infective Diseases” and “Digestive System” pathways significantly increased in 2nd instars. As for the effect of environment, the connection between living environment and the intestinal microbiome started to manifest after initial molting. Unexpectedly, initial feeding treatment slightly affected the intestinal microbiome of T. tridentatus in the early life stage, whereas the effect of initial molting was significant. The present study provided the first insight into the gut microbiota of T. tridentatus, and the findings led a new sight to explain what guide the change of gut microbiota.

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Data Availability

Sequence data that supports the findings of this study have been deposited in the National Center for Biotechnology Information (NCBI) with the BioProject accession number PRJNA624975 and PRJNA623068. Other datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This research was supported by a grant from 2017 Beihai City 13th 5-year Plan Marine Economic Innovation and Development Demonstration Project—Tachypleus Amebocyte Lysate and Chinese Horseshoe Crab Ecological Utilization Industry Chain Collaborative Innovation Project (Grant No.: Bhsfs006).

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  1. Fengze Miao and Zhonghao Zhao have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China

    Fengze Miao, Zhonghao Zhao, Youji Wang & Menghong Hu

  2. International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China

    Fengze Miao, Zhonghao Zhao, Youji Wang & Menghong Hu

  3. Guangxi Institute of Fisheries, Nanning, China

    Qiongzhen Li

  4. Tianjin Era Biology Technology Co., Ltd., Tianjin, China

    Jie Song

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  1. Fengze Miao
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Contributions

MH and JS conceived this project. FM, ZZ and QL performed the research. FM analyzed the data. FM, MH and YW wrote the paper. And all authors participated in the revision of this paper by providing comments and editing.

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Correspondence to Menghong Hu.

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Conflict of interest

The authors declare that they have no conflicts of interest. The cooperative enterprise in Tachypleus Amebocyte Lysate and Chinese Horseshoe Crab Ecological Utilization Industry Chain Collaborative Innovation Project (Grant No.: Bhsfs006)—Beihai Xinglong Biological Products Co., Ltd. has the "People's Republic of China Aquatic Wildlife Concession Capture Certificate" and the "People's Republic of China Aquatic Wildlife Domestication and Breeding License" issued by the Fisheries and Fisheries Administration of the Ministry of Agriculture. The adult horseshoe crabs used in our experiments were originally provided by Beihai Xinglong Biological Products Co., Ltd., and then were transported to Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding.

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Miao, F., Zhao, Z., Li, Q. et al. Impact of Initial Feeding and Molting on Tachypleus tridentatus Gut Microbiota. Curr Microbiol 77, 2847–2858 (2020). https://doi.org/10.1007/s00284-020-02108-x

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