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Preparation of three-dimensional graphene free-standing electrochemical sensor and its potential application in glucose determination

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Abstract

Lightweight, flexible electrodes based on three-dimensional (3D) graphene have attracted increasing attention owing to their potential applications in many fields. In this work, we developed 3D graphene through different methods and evaluated the potential of these materials as free-standing electrodes. Finally, the 3D graphene synthesized by hydrothermal gelation with cobalt ions as a cross-linking agent had the best electrochemical sensor performance. With our focus on practical applications, we further modified polydopamine and concanavalin A onto the 3D graphene to make it suitable for the detection of glucose. Under the optimum conditions, the impedance change in the free-standing electrode and the concentration of glucose have a good linear relationship in the range of 0.05–0.35 μmol/L and a limit of detection of 0.01 μmol/L. The electrochemical biosensor exhibited good sensitivity, selectivity, reproducibility, and stability. The fabricated biosensor was employed for the detection of glucose in mouse serum samples and provided satisfactory results. Therefore, the synthesized 3D graphene offers a new opportunity for immobilizing other biomolecules and fabricating biosensors.

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Preparation of functionalized three-dimensional graphene free-standing electrochemical sensor.

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Acknowledgements

We are grateful for financial support from the National Natural Science Foundation of China (no. 31000831), the Natural Science Foundation of Heilongjiang Province of China (C2016033), and the Harbin Outstanding Academic Leaders Plan (2014RFXXJ113).

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Authors and Affiliations

  1. Internal Trade Food Science and Technology (Beijing) Co., Ltd, No. 4 Road, Future Science and Technology Park South, Beiqijia, Changping, Beijing, 102209, People’s Republic of China

    Jia Liu

  2. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No. 92 West Dazhi Street, Harbin, 150001, Heilongjiang Province, People’s Republic of China

    Xingchen Zhai, Pan Chen & Xin Yang

  3. Consumer Testing Technology Co., Ltd., No. 7, 4th North Industry Road, Songshan Lake National High-Tech Industrial Development Zone, Dongguan, 523808, People’s Republic of China

    Weiqiang Cao

  4. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    A. M. Abd El-Aty

  5. Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240, Erzurum, Turkey

    A. M. Abd El-Aty & Ahmet Hacımüftüoğlu

Authors
  1. Xingchen Zhai
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  2. Pan Chen
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  3. Weiqiang Cao
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  4. Jia Liu
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  5. A. M. Abd El-Aty
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  6. Ahmet Hacımüftüoğlu
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  7. Xin Yang
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Correspondence to Jia Liu or Xin Yang.

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Zhai, X., Chen, P., Cao, W. et al. Preparation of three-dimensional graphene free-standing electrochemical sensor and its potential application in glucose determination. J IRAN CHEM SOC 17, 2845–2853 (2020). https://doi.org/10.1007/s13738-020-01961-8

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  • DOI: https://doi.org/10.1007/s13738-020-01961-8

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