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Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis

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Abstract

L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn2+. Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.

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Funding

This work was supported by the National Forestry Science and Technology Promotion Project [(2016)41] and the Key Research and Development Program of Jiangsu (BE2015759).

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Authors and Affiliations

  1. The Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China

    Zongren Guo, Liangkun Long & Shaojun Ding

  2. Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, Nanjing Forestry University, Nanjing, 210037, China

    Zongren Guo, Liangkun Long & Shaojun Ding

  3. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China

    Zongren Guo, Liangkun Long & Shaojun Ding

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  1. Zongren Guo
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Correspondence to Shaojun Ding.

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Guo, Z., Long, L. & Ding, S. Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis. Appl Biochem Biotechnol 192, 935–951 (2020). https://doi.org/10.1007/s12010-020-03380-0

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