Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes

Juan Chen; Jinlu Tang; Hong-Min Meng; Zhuo Liu; Lin Wang; Xin Geng; Yanan Wu; Lingbo Qu; Zhaohui Li

doi:10.1039/D0CC02337J

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Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes†

Juan Chen,‡^a Jinlu Tang,‡^b Hong-Min Meng,

*^a Zhuo Liu,^a Lin Wang,^c Xin Geng,^a Yanan Wu,^a Lingbo Qu^a and Zhaohui Li

*^a

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* Corresponding authors

^a College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, P. R. China
E-mail: hmmeng2017@zzu.edu.cn, zhaohui.li@zzu.edu.cn

^b School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, P. R. China

^c The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, P. R. China

Abstract

Here, we develop a facile split aptamer-based system for the amplified, specific and wash-free detection of exosomes in situ assisted by a target-induced hybridization chain reaction (HCR). This design was successfully used to detect target exosomes in a bio-matrix and distinguish cancer patients from healthy individuals.

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Article information

DOI: https://doi.org/10.1039/D0CC02337J
Article type: Communication
Submitted: 31 Mar 2020
Accepted: 01 Jul 2020
First published: 01 Jul 2020

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Chem. Commun., 2020,56, 9024-9027

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Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes

J. Chen, J. Tang, H. Meng, Z. Liu, L. Wang, X. Geng, Y. Wu, L. Qu and Z. Li, Chem. Commun., 2020, 56, 9024 DOI: 10.1039/D0CC02337J

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Chemical Communications

Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes†

Abstract

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Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes

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