Skip to main content
SpringerLink
Log in

A New Strategy of Overcoming both Matrix Effect and Shortage of Reference Standards for Determination of Multi-components in the Rhizomes of Alpinia officinarum Hance Using UHPLC-MS/MS with Single Exogenous Internal Standard

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

Alpinia officinarum Hance is a medicine and food dual purposes plant. Its rhizomes have been widely used as food and medicine in Asia, Africa, and Europe for high dietary values and therapeutic benefits. Phytochemical studies showed that flavonoids and diarylheptanoids are two groups of characteristic and bioactive components in the rhizomes of A. officinarum. However, comprehensive quality assessment of the rhizomes of A. officinarum is usually ignored for the shortages of reference standards of bioactive constituents. In this work, a new strategy of using curcumin-d6 as single exogenous internal standard was developed to overcome both matrix effect and shortage of reference standards for determination of seven quality markers (galangin, kaempferol, kaempferide, rac-pinocembrin, pinobanksin, dihydroyashabushiketol, 1,7-diphenyl-4-en-3-heptanone) in the rhizome of A. officinarum based on UHPLC-MS/MS method. Chromatographic separation was carried out on a C18 reverse-phase column with gradient elution. Mass spectrometric detection was accomplished using multiple-reaction-monitoring mode. The method developed was fully validated in terms of LOD, LOQ, linearity, precision, accuracy, and stability, and the results obtained by the method new developed were also compared with those obtained by conventional external standard method. Finally, the new developed analytical strategy was successfully applied to determine seven Q-markers in fifteen batches of rhizomes of A. officinarum. The newly developed method was useful for quality assessment of the rhizomes of A. officinarum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abubakar IB, Malami I, Yahaya Y, Sule SM (2018) A review on the ethnomedicinal uses, phytochemistry and pharmacology of Alpinia officinarum Hance. J Ethnopharmacol 224:45–62

    Article  CAS  Google Scholar 

  • Basri AM, Taha H, Ahmad N (2017) A review on the pharmacological activities and phytochemicals of Alpinia officinarum (galangal) extracts derived from bioassay-guided fractionation and isolation. Pharmacogn Rev 11:43–56

    Article  CAS  Google Scholar 

  • Chen P, Guo X, Liang H, Feng Y (2008) Analysis of the active constituents in Alpinia officinarum Hance. J Guangdong College Pharm 24:462–464

    CAS  Google Scholar 

  • De Nicolò A, Cantù M, D'Avolio A (2017) Matrix effect management in liquid chromatography mass spectrometry: the internal standard normalized matrix effect. Bioanalysis 9:1093–1105

    Article  CAS  Google Scholar 

  • Honmore VS, Rojatkar SR, Nawale LU, Arkile MA, Khedkar VM, Natu AD, Sarkar D (2016) In vitro and ex vivo antitubercular activity of diarylheptanoids from the rhizomes of Alpinia officinarum Hance. Nat Prod Res 30:2825–2830

    Article  CAS  Google Scholar 

  • Hou JJ, Wu WY, da J, Yao S, Long HL, Yang Z, Cai LY, Yang M, Liu X, Jiang BH, Guo DA (2011) Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard. J Chromatogr A 1218:5618–5627

    Article  CAS  Google Scholar 

  • Hou JJ, Wu WY, Liang J, Yang Z, Long HL, Cai LY, Fang L, Wang DD, Yao S, Liu X, Jiang BH, Guo DA (2014) A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui. J Pharm Biomed Anal 88:321–330

    Article  CAS  Google Scholar 

  • Kunati SR, Yang S, William BM, Xu Y (2018) An LC–MS/MS method for simultaneous determination of curcumin, curcumin glucuronide and curcumin sulfate in a phase II clinical trial. J Pharm Biomed Anal 156:189–198

    Article  CAS  Google Scholar 

  • Lan X, Wang W, Li Q, Wang J (2016) The natural flavonoid pinocembrin: molecular targets and potential therapeutic applications. Mol Neurobiol 53:1794–1801

    Article  CAS  Google Scholar 

  • Lee HN, Shin SA, Choo GS, Kim HJ, Park YS, Kim BS, Kim SK, Cho SD, Nam JS, Choi CS, Che JH, Park BK, Jung JY (2018) Anti-inflammatory effect of quercetin and galangin in LPS-stimulated RAW264.7 macrophages and DNCB-induced atopic dermatitis animal models. Int J Mol Med 41:888–898

    PubMed  CAS  Google Scholar 

  • Li ZY, Sun DM, Zhang JJ (2010) Determination of galangin and kaempferol in Rhizoma Alpiniae Officinarum by HPLC. Chin J Tradit Chin Med Pharm 25:1368

    CAS  Google Scholar 

  • Lim TK (2016) Alpinia officinarum. In: Lim TK (ed) Edible medicinal and non-medicinal plants, Modified stems, roots, vol 12. Bulbs. Springer International Publishing, Cham, pp 178–195

    Google Scholar 

  • Lin LY, Peng CC, Yeh XY, Huang BY, Wang HE, Chen KC, Peng RY (2015) Antihyperlipidemic bioactivity of Alpinia officinarum (Hance) Farw Zingiberaceae can be attributed to the coexistance of curcumin, polyphenolics, dietary fibers and phytosterols. Food Funct 6:1600–1610

    Article  CAS  Google Scholar 

  • Lin L, Liu X, Zhao M (2018) Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats. J Funct Foods 50:26–36

    Article  CAS  Google Scholar 

  • Liu Z, Sang S, Hartman TG, Ho CT, Rosen RT (2005) Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and Electrochemical Detection. Phytochem Anal 16:252–256

  • Liu D, Liu YW, Guan FQ, Liang JY (2014a) New cytotoxic diarylheptanoids from the rhizomes of Alpinia officinarum Hance. Fitoterapia 96:76–80

    Article  CAS  Google Scholar 

  • Liu Y, Wang X, Liu Y, Di X (2014b) Simultaneous determination of four flavonoids in Alpinia officinarum hance by RP-HPLC. J Shenyang Pharm Univ 31:13–16

    Google Scholar 

  • National Commission of Chinese Pharmacopoeia (2015) Pharmacopoeia of the People’s republic of China, vol. I. China Medical Science Press, Beijing, pp 287–288

    Google Scholar 

  • National Health Commission of the People’s Republic of China (2002) Notice of the Chinese Ministry of Health on Further Regulating the Management of Raw Materials of Healthy Foods, Wei Fa Jian Fa No. 51, 2002. < http://www.nhc.gov.cn/sps/s3593/200810/a298f342c464475b923bcdc3c9d0575a.shtml> Accessed 14.02.2020

  • Ning Z, Liu Z, Song Z, Zhao S, Dong Y, Zeng H, Shu Y, Lu C, Liu Y, Lu A (2016) A single marker choice strategy in simultaneous characterization and quantification of multiple components by rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS). J Pharm Biomed Anal 124:174–188

    Article  CAS  Google Scholar 

  • Peng G, Zhou G, Qin H (2014) Simultaneous determination of six compounds in Alpinia officinarum by HPLC. Chin J Pharm Anal 34:966–970

    CAS  Google Scholar 

  • Theodoridis GA, Gika HG, Want EJ, Wilson ID (2012) Liquid chromatography–mass spectrometry based global metabolite profiling: a review. Anal Chim Acta 711:7–16

    Article  CAS  Google Scholar 

  • Wang W, Ma X, Guo X, Zhao M, Tu P, Jiang Y (2015) A series of strategies for solving the shortage of reference standards for multi-components determination of traditional Chinese medicine, Mahoniae caulis as a case. J Chromatogr A 1412:100–111

    Article  CAS  Google Scholar 

  • Zhang JQ, Wang Y, Li HL, Wen Q, Yin H, Zeng NK, Lai WY, Wei N, Cheng SQ, Kang SL, Chen F, Li YB (2015) Simultaneous quantification of seventeen bioactive components in rhizome and aerial parts of Alpinia officinarum Hance using LC-MS/MS. Anal Methods 7:4919–4926

    Article  CAS  Google Scholar 

  • Zhang G, Zhao L, Zhu J, Feng Y, Wu X (2018) Anti-inflammatory activities and glycerophospholipids metabolism in KLA-stimulated RAW 264.7 macrophage cells by diarylheptanoids from the rhizomes of Alpinia officinarum. Biomed Chromatogr 32:e4094

    Article  CAS  Google Scholar 

  • Zhao X, Chen X, Geng L, Bi K (2009) HPLC simultaneous determination of 3 flavonoids in Alpinia officinarum Hance. Chin J Pharm Anal 29:2036–2039

    CAS  Google Scholar 

  • Zhu C, Li X, Zhang B, Lin Z (2017) Quantitative analysis of multi-components by single marker—a rational method for the internal quality of Chinese herbal medicine. Integr Med Res 6:1–11

    Article  Google Scholar 

Download references

Funding

This work was supported by the National Natural Science Foundation of China (No. 31400302), the China Scholarship Council (No. 201708420116), and Qingmiao Project of Hubei university of Chinese Medicine (No. 2015ZXX015).

Author information

Authors and Affiliations

  1. Hubei Key Laboratory of Resource and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, Hubei, People’s Republic of China

    Dan Liu & Xiaoqing Ma

  2. Department of Chemistry, Cleveland State University, Cleveland, OH, 44115, USA

    Dan Liu, Gang Xu & Yan Xu

Authors
  1. Dan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoqing Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Dan Liu and Yan Xu contributed equally to the study conception and design. Dan Liu and Gang Xu developed methodology and performed data acquisition. Xiaoqing Ma collected the materials. The first draft of the manuscript was written by Dan Liu and Yan Xu make critical corrections. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Dan Liu or Yan Xu.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethics Approval

This article does not contain any studies with human or animal subjects.

Consent to Participate

Not applicable.

Consent for Publication

Not applicable

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 30.4 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, D., Xu, G., Ma, X. et al. A New Strategy of Overcoming both Matrix Effect and Shortage of Reference Standards for Determination of Multi-components in the Rhizomes of Alpinia officinarum Hance Using UHPLC-MS/MS with Single Exogenous Internal Standard. Food Anal. Methods 13, 1867–1878 (2020). https://doi.org/10.1007/s12161-020-01802-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-020-01802-7

Keywords

Search

Navigation