Sarcoplasmic Reticulum-Mitochondria Kissing in Cardiomyocytes: Ca²⁺, ATP, and Undisclosed Secrets

Introduction

In muscle cells, the sarcoplasmic reticulum (SR) is a specialized, differentiated domain of the endoplasmic reticulum (ER), generated from a reorganization of the ER membranes during myogenesis (Michalak and Opas, 2009). SR consists of a network of membranes, closely associated with the myofibrils, specialized in the regulation of Ca²⁺ transport and thus the control of excitation-contraction coupling (ECC). Though ER and SR membranes/lumen are continuous, protein distribution is polarized, with completely different kinetics of Ca²⁺ uptake and release between the two compartments. As an example, ryanodine receptors (RyRs) and calsequestrin are enriched in the SR, whereas inositol-trisphosphate receptors (IP3Rs) and calreticulin in the ER (Michalak and Opas, 2009). Importantly, SR can be divided into two distinct domains: the longitudinal SR (formed by interconnected tubules surrounding myofibrils) and junctional SR (jSR) (formed by the terminal cisternae of the longitudinal SR juxtaposed with TTs) (Figure 1).

Figure 1. The cartoon represents the structural organization of TT-SR-mitochondria association within a cardiomyocyte. On the right, note how Ca²⁺ microdomains, generated at TT-JSR interface, can reach the edges of juxtaposed mitochondria, favoring mitochondrial Ca²⁺ uptake through the MCUC. See the text for details on the specific Ca²⁺ channel/pump distribution.

As to mitochondria, in adult cardiomyocytes, they are densely packed between jSR and parallel tubules of longitudinal SR, tethered to both SR domains. Specifically, seminal ultrastructural studies revealed, by electron microscopy (EM), a highly orderly organelle juxtaposition between SR and mitochondria, maintained by electron-dense filamentous tethers, whose length is approximately 10 nm (Ramesh et al., 1998; Sharma et al., 2000; Boncompagni et al., 2009; Hayashi et al., 2009). The molecular identity of these tethers is not completely clear (see also below). However, ER-mitochondria “bridges” in non-cardiac cells have been reported to be composed by proteins, to extend for 6–15 nm and to frequently occur in clusters, where they are spaced 13–22 nm apart (Csordás et al., 2006). Importantly, in mice, SR-mitochondria association is generated after birth in a developmentally regulated process, in which mitochondria progressively migrate from a random, predominantly sub-sarcolemma distribution, to their definitive positioning between the myofibrils. In this process, which parallels the maturation of both the primitive TT system and of SR, mitochondria gradually tether to jSR (Franzini-Armstrong, 2007; Boncompagni et al., 2009), with a progressive increase in the occurrence of tethers and organelle contacts.

In the early postnatal days, SR-mitochondria contacts are scarcely observed, possibly suggesting a non-essential role for cell viability; yet, their developmentally regulated appearance hints a structural and/or functional advantage. However, while the ultrastructural features of this coupling have been well defined in the heart, on the other hand (and in contrast with other tissues) its functional significance is still debated. Below, we briefly review these aspects.

Structural Organization of SR-Mitochondria Coupling

Within SR, the specific distribution of the Ca²⁺ releasing channels (RyR2 in cardiomyocytes) and the sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA) argues against a significant contribution of mitochondrial localization to the process of mitochondrial Ca²⁺ uptake (see below). Conversely, some experimental evidence suggests this latter could take advantage of the strategic organelle positioning. Indeed, RyRs do not homogenously distribute in the SR membrane, but are specifically recruited on the jSR subdomain facing the TTs (Figure 1). Here, they are closely associated with the TT-located L-type voltage-gated Ca²⁺ channels (VOCCs), originating the “Ca²⁺ releasing unit” (CRU). In these interface regions (dyadic clefts), TTs and jSR juxtapose with a gap of ∼10–15 nm, where electron-dense structures, representing RyR “feet,” are observed (Eisner et al., 2017). Upon an action potential (AP), TTs guarantee an almost simultaneous spreading of sarcolemma depolarization, inducing Ca²⁺ entry through VOCCs. This Ca²⁺ influx generates local Ca²⁺ microdomains, intense enough to stimulate the Ca²⁺-mediated opening of RyRs, a process called “Ca²⁺-induced-Ca²⁺-release” (CICR). In turn, RyR opening triggers a massive Ca²⁺ release from the SR into the dyadic cleft, promptly spreading in the whole cell and ultimately responsible for cell contraction. Of note, mitochondria are excluded from the dyadic clefts, being tethered to jSR on the opposite side (Sharma et al., 2000). This implies that SR lumen separates RyRs from mitochondria (Figure 1). Therefore, before reaching the outer mitochondrial membrane (OMM), Ca²⁺ must diffuse around the jSR cisternae, dissipating part of the steep concentration gradient generated in the nearest CRU proximity. The distance between RyR feet located at the edge of jSR and the OMM of the closest mitochondria is ∼35–40 nm, whereas Ca²⁺, released in the middle of the dyadic cleft, must cover an additional distance to firstly reach the edge of the junction (∼230 nm), and then the OMM (Sharma et al., 2000). This range of distances is considerably higher than that commonly observed in different, non-muscular cell types, where ER-mitochondria Ca²⁺ tunneling is guaranteed by the very close apposition between the two organelles (∼10–25 nm; Filadi et al., 2017c) and by the strategic interaction between IP3Rs on the ER and voltage-dependent-anion-channels (VDACs) on the OMM (Szabadkai et al., 2006).

Sarco/endoplasmic reticulum Ca²⁺-ATPase is also excluded from the dyadic cleft (Fearnley et al., 2011), but is abundant in the longitudinal SR (which surround mitochondria; Greenstein and Winslow, 2011), promptly taking up Ca²⁺ that reaches these regions and further hindering mitochondrial Ca²⁺ uptake. Of note, the overall cytosolic Ca²⁺ peak reached during an AP is ∼1–3 μM (Fearnley et al., 2011). All these considerations argue against the possibility that, during physiological ECC, mitochondria are exposed to sufficiently high Ca²⁺ concentrations [Ca²⁺] to match the relatively low Ca²⁺ affinity of the mitochondrial Ca²⁺ uniporter complex (MCUC). This latter is endowed with a Kd for Ca²⁺ of ∼15 μM and is composed by the pore-forming subunits (MCU and/or the dominant negative isoform MCUb), by the essential component EMRE (a 10 kDa protein spanning the inner mitochondrial membrane, IMM) and by additional regulatory subunits residing in the mitochondrial intermembrane space (MICU1, MICU2 and, in certain tissues, MICU3; reviewed in Mammucari et al., 2018). However, the peculiar ultrastructure of cardiac organelle juxtaposition may overcome these apparent odds. Indeed, at the dyadic cleft: (a) the very close jSR-TT apposition and the small volume of the cleft (Hayashi et al., 2009), strongly favor lateral Ca²⁺ diffusion along the junction, limiting its radial dispersion; (b) several RyR feet fuel CICR and amplify Ca²⁺ microdomain, up to 100 μM (Fearnley et al., 2011); (c) the absence of SERCA (and, possibly, the local depletion of cytosolic Ca²⁺ buffers) enhance Ca²⁺ spreading; (d) longitudinal SR elements, frequently connecting the two jSR cisternae located at the opposite sides of a TT (Boncompagni et al., 2009), may limit Ca²⁺ diffusion outside of the junction (Figure 1). Thus, the specific arrangement of juxtaposed membranes and Ca²⁺ handling proteins may create a “railway” enhancing Ca²⁺ diffusion over relatively long distances, from the junction to the nearby mitochondria, before further spreading in the whole cell. This would imply an heterogeneous Ca²⁺ uptake among mitochondrial population (Pacher et al., 2002; Lu et al., 2013), with those organelles located in closer apposition with the CRU experiencing the highest Ca²⁺ rises.

Interestingly, along the IMM of cardiac mitochondria, the distribution of both the MCUC and the Na⁺/Ca²⁺ exchanger (NCLX, the major mitochondrial Ca²⁺ efflux mechanism in excitable cells; Palty et al., 2010) is not homogenous. Intriguingly, a strategic positioning of MCUC toward areas juxtaposed with jSR has been reported (De La Fuente et al., 2016), whereas NCLX is actively excluded from these regions, but abundantly expressed in the sub-mitochondrial fraction free of contacts (De La Fuente et al., 2018). This biased positioning of the uptake and efflux machineries has been suggested to enhance the efficiency of the excitation-energetics coupling. Indeed, it favors the generation of mitochondrial Ca²⁺ signals capable of sustaining mitochondrial metabolism (see below), while minimizing the energetic cost (in terms of transient mitochondrial depolarization) inevitably associated with mitochondrial Ca²⁺ uptake (De La Fuente et al., 2018). The mechanisms underlying this sub-mitochondrial protein distribution are unknown. However, in rat heart, it has been observed that the OMM-IMM contact points are aligned with those between jSR and mitochondria (Garcia-Perez et al., 2011). This possibly suggests that the OMM-IMM contacts may work as a platform for the anchorage of specific structures (such as the tethers connecting the OMM with SR, composed by proteins possibly anchored to particular lipids) and the recruitment/exclusion of specific protein complexes (such as MCUC or NCLX).

Overall, the tightly regulated organization of the machineries that control mitochondrial Ca²⁺ dynamics, specifically observed in cardiomyocytes, argues in favor of the key importance of mitochondria-jSR apposition and, more in general, of mitochondrial Ca²⁺ signaling, for heart physiology.

SR-Mitochondria Tethers

While the composition of the protein complexes mediating ER-mitochondria physical/functional tethering has been intensively studied in non-muscular cells (Filadi et al., 2017c), the molecular identity of SR-mitochondria tethers has been poorly investigated in the heart. Most studies focused on mitofusin-2 (MFN2), an OMM protein (in part present also at the ER membranes) involved in mitochondrial fusion. The role of this protein as a putative ER-mitochondria tether has been a matter of intense debate, suggesting either a pro-tethering (de Brito and Scorrano, 2008; Naon et al., 2016) or an anti-tethering (Cosson et al., 2012; Filadi et al., 2015, 2017b) function. Similarly, in the heart, contradictory results have been obtained. The cardiomyocyte-specific deletion of the Mfn2 gene after birth has been observed to reduce the length of SR-mitochondria contacts by ∼30%, but the distance between jSR and the OMM was not significantly altered (Chen et al., 2012). In isolated cardiomyocytes from this mouse model, upon isoproterenol exposure, higher cytosolic and lower mitochondrial Ca²⁺ rises, associated with a reduced stimulation of the Krebs cycle, were observed. On the other hand, in a different mouse model in which Mfn2 was specifically deleted in the embryonic heart, jSR-mitochondria apposition was found to be maintained, without significant differences in Ca²⁺ dynamics (Papanicolaou et al., 2011). Importantly, profound alterations of mitochondrial morphology were observed in both models. However, to the best of our knowledge, Mfn2 ablation has never been reported to completely disrupt SR-mitochondria contacts, nor to decrease the presence of electron-dense filamentous bridges between the two organelles, possibly suggesting that MFN2 is not essential for their formation. Likely, multiple tethers may exist and exert redundant activities.

For instance, recently, the cardiac-specific downregulation of protein-tyrosine-phosphatase-interacting-protein 51 (PTPIP51) has been demonstrated to reduce the extension of SR-mitochondria contacts, while increasing organelle distance, reducing caffeine-induced SR to mitochondria Ca²⁺ transfer and protecting from ischemia-reperfusion damage (Qiao et al., 2017). The opposite effects have been observed upon PTPIP51 overexpression, suggesting that it may participate in contact stabilization, as reported for ER-mitochondria tethering (Stoica et al., 2014). Similarly, FUN14-domain-containing 1 (FUNDC1) on the OMM has been shown to bind IP3R2 on ER membranes, maintaining structural organelle coupling, favoring IP3-linked ER-mitochondria Ca²⁺ shuttling and sustaining cardiac functionality (Wu et al., 2017). However, whether FUNDC1 activity is limited to ER-mitochondria association, or extend also to the SR-mitochondria one, is not completely clear. Indeed, whether specialized SR-mitochondria tethers exist, or they are completely redundant with those mediating ER-mitochondria apposition, remains an unanswered question.

Functional Evidence of SR-Mitochondria Ca²⁺ Coupling

As far as SR-mitochondria Ca²⁺ transfer in the heart is concerned, in the microdomain that bathes the ends of the mitochondria closer to the CRUs, mathematical models calculated a [Ca²⁺] of ∼10-20 μM (Williams et al., 2011, 2013), lasting for ∼10 ms (Cheng and Lederer, 2008). In rat neonatal cardiomyocytes, the existence of Ca²⁺ hot-spots during spontaneous Ca²⁺ oscillations has been demonstrated by an OMM-targeted FRET-based Ca²⁺ probe (Drago et al., 2012). Upon caffeine-induced RyR stimulation, a prompt mitochondrial Ca²⁺ uptake was observed in permeabilized rat ventricular cardiomyocytes, with a marginal reduction when the fast Ca²⁺ buffer BAPTA was included in the experimental buffer (Sharma et al., 2000). Similarly, in permeabilized cardiac H9c2 cells stimulated with caffeine, it has been calculated that ∼25% of the Ca²⁺ released from SR is taken up by mitochondria (Pacher et al., 2000), further suggesting that they are exposed to sustained Ca²⁺ microdomains. It is worth noting, however, that caffeine stimulation triggers a higher and slower cytosolic Ca²⁺ rise, compared with the physiological Ca²⁺ oscillations observed during ECC, thereby differently impacting on the machinery that mediate mitochondrial Ca²⁺ uptake.

Whether and how mitochondria decode cytosolic Ca²⁺ transients during ECC is debated. Contrasting data have been reported, suggesting either smoothened, slow changes in steady-state mitochondrial Ca²⁺ levels (Miyata et al., 1991; Griffiths et al., 1997; Sedova et al., 2006), or rapid beat-to-beat oscillations, closely following those in the cytosol (Chacon et al., 1996; Robert et al., 2001; Bell et al., 2006; Maack et al., 2006; Garcia-Perez et al., 2008; Drago et al., 2012). The controversy may depend on both the specific Ca²⁺ probes (chemical dyes or genetically encoded indicators) and types of stimulations (electrical, β-adrenergic or spontaneous contraction) used in the different studies. This topic has been extensively reviewed and the interested readers are referred to a comprehensive contribution (De la Fuente and Sheu, 2019). Recently, however, by using the FRET-based Cameleon Ca²⁺ probe 4mtD3cpv expressed in cultured rat cardiomyocytes, it has been demonstrated that, upon a low frequency electrical stimulation (0.1 Hz), mitochondria promptly take up Ca²⁺ (rise time ∼49 ms), whereas their Ca²⁺ efflux is relatively slow (decay half time ∼1.17 s) (Wüst et al., 2017). At higher frequency stimulation, this asymmetry results in a progressive rise in mitochondrial [Ca²⁺], until a new balance between uptake and efflux is reached, yet maintaining a beat-to-beat oscillatory pattern (Wüst et al., 2017). These results confirmed those previously observed in rabbit ventricular myocytes, where the repetitive rise in mitochondrial [Ca²⁺] was observed to be higher and to occur earlier in the pool of mitochondria closer to jSR (Lu et al., 2013). Importantly, the observation that the efflux kinetics are much slower than the uptake ones implies that beat-to-beat mitochondrial Ca²⁺ oscillations might be more easily observed (and could have a larger physiological relevance) in organisms endowed with lower heart rates, such as humans [heart rate ∼1–1.5 Hz; note the match with the reported half time of mitochondrial Ca²⁺ efflux, ∼1.17 s (Wüst et al., 2017)].

Physiological Significance of Mitochondrial Ca²⁺ Uptake in Cardiomyocytes

As to the possible significance of mitochondrial Ca²⁺ transients in the heart, they have been consistently suggested to stimulate ATP synthesis, thus matching the increased energy demand upon cell contraction (Yaniv et al., 2010; Dorn and Maack, 2013). Indeed, in the mitochondrial matrix, Ca²⁺ stimulates Krebs cycle, thereby enhancing the synthesis of NADH and FADH₂, in turn fuelling the electron transport chain (ETC) and, ultimately, ATP synthase (reviewed in Rossi et al., 2019). Moreover, in the mitochondrial-intermembrane-space, Ca²⁺ stimulates the activity of different metabolite transporters, further enhancing mitochondrial metabolism (Rossi et al., 2019). Last, but not least, the Ca²⁺-mediated stimulation of the Krebs cycle increases the mitochondrial antioxidative capacity by enhancing the synthesis of NADPH, in turn essential for the activity of key H₂O₂-eliminating enzymes, such as glutathione peroxidase (Kohlhaas et al., 2017). Importantly, reactive oxygen species (ROS, which, in mitochondria, are mainly generated as by-products of oxidative phosphorylation) can act as signaling molecules and regulate the activity of different Ca²⁺ handling protein, including SERCA and RyR2 (Feno et al., 2019). Therefore, a complex crosstalk exists between Ca²⁺ and ROS signaling, in which SR-mitochondria juxtaposition likely plays a key role, as observed for ER-mitochondria interface and IP3R-mediated Ca²⁺ transfer (Booth et al., 2016).

An increase in the heart workload is associated with an increase in the frequency and amplitude of cytosolic Ca²⁺ transients, enhancing myofilament contraction. In turn, this triggers ATP consumption, to allow both muscle relaxation and recovery of basal cytosolic Ca²⁺ levels. Therefore, the capacity of mitochondria to decipher transient cytosolic Ca²⁺ oscillations would represent an elegant mechanism to match, in real-time, the acute ATP demand of the beating heart. On the other hand, slower and smoothened mitochondrial Ca²⁺ dynamics would not provide mitochondria with a sufficient temporal resolution to face acute fluctuations of cell energy need. Interestingly, while the close juxtaposition of mitochondria with jSR favors mitochondrial Ca²⁺ uptake (see above), the organelle coupling with longitudinal SR may provide a structural advantage to fuel SERCA (which is abundant in this SR domain) with both mitochondria-derived ATP (Wilding et al., 2006) and Ca²⁺ released through NCLX after mitochondrial Ca²⁺ uptake (as suggested in different cell models; Malli et al., 2005; Poburko et al., 2009). Overall, evidence has been provided suggesting that mitochondrial Ca²⁺ signals are important for ATP synthesis in cardiomyocytes (Maack et al., 2006; Garcia-Perez et al., 2008; Liu and O’Rourke, 2009; Chen et al., 2012; Bertero and Maack, 2018; Wescott et al., 2019), but whether this regulation is carried out on a beat-to-beat basis is not clear. A defective SR-mitochondria coupling, reducing organelle Ca²⁺ exchange and maximal mitochondrial respiration, while increasing mitochondrial ROS, has been reported to associate with heart aging (Fernandez-Sanz et al., 2014). Recently, a critical role for IP3R-mediated (but not for RyR-mediated) SR-mitochondria Ca²⁺ transfer on mitochondrial ATP synthesis has been reported in mouse ventricular myocytes (Seidlmayer et al., 2016), suggesting that different Ca²⁺ signaling pathways may differently impact on mitochondrial metabolism.

In addition to the role played in the modulation of cardiomyocyte bioenergetics, mitochondrial Ca²⁺ uptake has been suggested to buffer part of the Ca²⁺ released from SR during ECC, thereby shaping cytosolic Ca²⁺ oscillations and modulating cell contraction. In rat neonatal cardiomyocytes, MCU downregulation by siRNA (decreasing mitochondrial Ca²⁺ uptake) increases cytosolic Ca²⁺ peaks and contraction during spontaneous Ca²⁺ pacing, whereas MCU overexpression triggers the opposite effects (Drago et al., 2012). Similarly, in adult cardiomyocytes from an MCU-cKO mouse model, isoproterenol-induced cytosolic Ca²⁺ transients were higher compared with WT animals (Luongo et al., 2015). Finally, upon field stimulation, higher diastolic and systolic cytosolic [Ca²⁺], associated with lower ATP levels, were observed in ventricular myocytes from transgenic mice selectively expressing in myocardium a dominant-negative form of MCU (Rasmussen et al., 2015). However, contradictory results are present in literature. Indeed, earlier studies failed to detect a significant impact of mitochondrial Ca²⁺ uptake on cytosolic Ca²⁺ transients in cardiomyocytes (Andrienko et al., 2009; Williams et al., 2013; Boyman et al., 2014). As discussed above, differences between species or experimental setups may underlie part of the discrepancies. Importantly, it is worth noting that cytosolic Ca²⁺ dynamics, among other determinants, are deeply affected by SERCA activity, which controls both the amount of Ca²⁺ stored within SR and the speed/amplitude of Ca²⁺ re-uptake after release. SERCA activity, however, is quite sensitive to ATP availability (Tian and Ingwall, 1996). Therefore, the process of mitochondrial Ca²⁺ uptake may affect cytosolic Ca²⁺ transients not only directly (by buffering the cation), but also indirectly, by modulating ATP availability.

Above, we discussed the importance of mitochondrial Ca²⁺ signaling in cardiomyocytes. However, recent experimental evidence cast doubts on its overall significance. In particular, three different mouse models, in which whole organism or cardiac mitochondrial Ca²⁺ accumulation was severely compromised by manipulating MCUC composition (Pan et al., 2013; Holmström et al., 2015; Kwong et al., 2015; Luongo et al., 2015; Rasmussen et al., 2015; Wu et al., 2015), displayed an almost normal heart functionality at rest. On the other hand, upon β-adrenergic stimulation, a reduced contractile responsiveness, associated with a decreased cardiac performance, was observed (Kwong et al., 2015; Luongo et al., 2015; Rasmussen et al., 2015). These results may suggest that MCU-mediated mitochondrial Ca²⁺ uptake is dispensable for basal cardiac activity, but is necessary upon acute increases of heart workload. The lack of gross alterations in the absence of MCU might be surprising. Importantly, however, the impact of mitochondrial Ca²⁺ uptake on heart performance could be species-specific, depending on the different heart rates and thus on the amplitude of the beat-to-beat Ca²⁺ oscillations in the mitochondrial matrix (see above). Moreover, it should be noted that, although the process of rapid mitochondrial Ca²⁺ uptake is severely (if not completely) compromised in these models, yet the resting mitochondrial Ca²⁺ levels are not deeply affected (Kwong et al., 2015). This may suggest the possible existence of additional (though less efficient) mitochondrial Ca²⁺ uptake mechanisms. For instance, a mitochondria-specific RyR1 (mRyR1), mediating Ca²⁺ uptake, has been reported to be expressed in the IMM of heart organelles and activated at low cytosolic [Ca²⁺] (Beutner et al., 2001, 2005). Though the existence and the physiological importance of mRyR1 is still controversial, it has been recently suggested to enhance ATP synthesis, by transferring Ca²⁺ from SR to mitochondria upon IP3-dependent cardiomyocyte stimulation (Seidlmayer et al., 2016).

Overall, the importance of a strictly regulated mitochondrial Ca²⁺ signaling is outlined by a series of evidences. NCLX deletion in adult mouse heart is lethal, inducing mitochondrial Ca²⁺ overload, activation of the mitochondrial permeability transition pore, necrotic cell death, and sudden heart failure (Luongo et al., 2017). Importantly, NCLX ablation is well tolerated when performed shortly after birth, hinting unknown compensatory adaptations in the maturing heart, and NCLX overexpression in mice was observed to protect from ischemia-reperfusion injury and reduce infarct size (Luongo et al., 2017). On the same line, in some (but not all) mouse models, MCU deletion has been demonstrated to protect from cardiac ischemia-reperfusion damage, by preventing mitochondrial Ca²⁺ overload (Kwong et al., 2015; Luongo et al., 2015). Moreover, the expression of the dominant negative MCU subunit (MCUb) is particularly abundant in the heart (Raffaello et al., 2013) and MCUC density and activity is very low (Fieni et al., 2012). In particular, MCU translation, regulated by microRNA-1 levels, decreases during postnatal cardiac growth, both in mice and humans, whereas MCUb expression increases in mice (Zaglia et al., 2017). The reason why, during cardiomyocyte maturation, the increase in SR-mitochondria association (Boncompagni et al., 2009) is paralleled by a decreased MCU expression (Zaglia et al., 2017) remains an outstanding question. It is tempting to speculate that, in mature heart cells, mitochondria develop specific toolkits to decipher low-level, basal Ca²⁺ fluctuations, while becoming particularly sensitive to Ca²⁺ overload. Interestingly, in physiologic and pathologic cardiac hypertrophy, MCU protein levels increase, a process prevented by β-blocker treatment (Zaglia et al., 2017). Furthermore, the expression of the MCUC regulatory subunit MICU2 is altered in patients with ventricular hypertrophy, and MICU2-KO mice display diastolic dysfunction, possibly associated with delayed cytosolic Ca²⁺ re-uptake and decreased cardiomyocyte relaxation (Bick et al., 2017).

Finally, in cardiomyocytes, cyclic AMP (cAMP) and Ca²⁺ signaling are well known to intersect and modulate with each other (reviewed in Filadi et al., 2017a). Interestingly, recently, in neonatal rat ventricular cardiomyocytes, the presence of a compartmentalized cAMP-PKA signal at the OMM has been demonstrated (Burdyga et al., 2018). Whether the narrow gap between SR and OMM might further shape this localized pathway will require further investigations.

Conclusion

In the last decade, the rapid growth in our knowledge of the mechanisms regulating the complex balance between mitochondrial Ca²⁺ signal and cell metabolism has tremendously increased our understanding of cardiomyocyte physiopathology, yet prompting additional and unpredicted questions. The intimate relationship between SR and mitochondria appears critical to regulate mitochondrial Ca²⁺ uptake. Nevertheless, whether mitochondria take advantage of their strategic positioning to efficiently detect Ca²⁺ microdomains on a beat-to-beat basis is still matter of debate. Species-specific differences, partly due to diverse heart rates, may dramatically impact on the complex balance between Ca²⁺ uptake/efflux dynamics, resulting in substantially different outcomes. Moreover, the lack of severe phenotypes in mice lacking key MCUC components suggests that additional routes, guaranteeing a sufficient, albeit minimal, Ca²⁺ signal in the mitochondrial matrix might exist. Alternatively, unknown compensatory mechanisms may occur (see for example, the dramatically different effect of NCLX deletion in adult vs newborn mouse heart; Luongo et al., 2017).

In addition to Ca²⁺ transfer, lipid metabolism is known to be modulated by ER-mitochondria tethering; yet, as far as SR-mitochondria juxtaposition is concerned, this aspect has been largely neglected in literature. Similarly, the impact of SR-mitochondria contacts on autophagy, as well as on the regulation of mitochondrial dynamics and morphology, has not been sufficiently explored in the heart. Clearly, an in-depth investigation of the molecules regulating SR-mitochondria coupling appears urgent to study these issues. The development of novel techniques, enabling to simultaneously measure with sufficient spatial and temporal resolution different parameters, will offer the opportunity to further raise the bar, allowing to precisely evaluate the impact of these pathways in different cardiac pathologies.

Author Contributions

MR and RF revised the literature, wrote and discussed the manuscript. All authors contributed to the article and approved the submitted version.

Funding

RF research was supported by the National Research Council (CNR) and by “Premio Scientifico 2018 DSB-CNR.”

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors would like to thank P. Pizzo and T. Pozzan for continuous support and P. Pizzo for discussion/critical reading of the manuscript.

References