Abstract
Abnormal metabolism of cancer cells leads to changes in the concentration of various components in the cell metabolic fluid, which is obviously different from that in normal cells. The two most representative parameters used for the early diagnosis of cancer are acidity (pH) and dissolved oxygen (DO) in the external environment of cancer cells. This study aimed to propose a dual-parameter detection method to simultaneously determine the pH and DO of cancer cell metabolic fluid based on the fluorescence spectrum of coumarin-modified CdSe quantum dots. Quantum dot is an important low dimensional semiconductor material with nanometer level. The coumarin-modified CdSe quantum dots can be ultraviolet-excited with an excitation light of wavelength 365 nm. The realization of detecting pH and DO was demonstrated by characterizing the fluorescence intensity at 625 nm produced by CdSe quantum dots and at 485 nm induced by coumarin upon excitation. The results of the experiment with phosphate-buffered saline showed that the detection limit of pH was 0.05 and that of DO was 0.04 mg/L. Based on this, the pH and DO of the extracellular environment of cancer and normal cells were tested and compared to verify the accuracy of the measurement.The method proposed in this study could realize the dual-parameter detection of the cell metabolic fluid with high measurement accuracy, high speed, and less loss of raw liquid. The method could also be applied to other related detection fields.
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10 August 2021
A Correction to this paper has been published: https://doi.org/10.1007/s11051-021-05231-3
References
Charnnok B, Suksaroj T, Boonswang P, Chaiprapat S (2013) Oxidation of hydrogen sulfide in biogas using dissolved oxygen in the extreme acidic biofiltration operation. J. Bioresour Technol 131:492–499
Chu CS, Lin CA (2014) Optical fiber sensor for dual sensing of temperature and oxygen based on PtTFPP/CF embedded in sol-gel matrix. J Sensors Actuators B 195:259–265
Dai WY, Sun L (2007) Determination of dissolved oxygen in water. J Anhui Agric Sci Bull 13(19):77–79
Felber AE, Dufresne M-H, Leroux JC (2012) pH-sensitive vesicles, polymeric micelles, and nanospheres prepared with polycarboxylates. J Adv Drug Deliv Rev 64(11):979–992
Gu B, Yin MJ, Zhang AP, Qian JW, He S (2009) Low-cost high-performance fiber-optic pH sensor based on thin-core fiber modal interferometer. J Optics Express 17(25):22296–22302
Koppenol WH, Bounds PL, Dang CV (2011) Otto Warburg's contributions to current concepts of cancer metabolism. J Nature Rev Cancer 11(5):325–337
Redy-Keisar O, Huth K, Vogel U, Lepenies B, Seeberger PH, Haag R, Shabat D (2015) Enhancement of fluorescent properties of near -infrared dyes using clickable oligoglycerol dendrons. J Org Biomol Chem 13:4727–4732
Shao Q, Biel MA, Ashkenazi S (2014) Noninvasive tumor oxygen imaging by photoacoustic lifetime imaging integrated with photodynamic therapy. J Proc SPIE Int Soc Opt Eng 8931(1):1115–1121
Siegel RL, Miller KD, Jemal A (2020) Cancer statistics, 2020. CA A Cancer J Clin 70(1):7–30
Tang YH, Pei LZ, Zhao XQ (2011) Introduction to nanomaterials. M. Changsha: Hunan university press
Tian Y, Shumway BR, Cody Youngbull A, Li Y, Jen AKY, Johnson RH, Meldrum DR (2010) Dually fluorescent sensing of pH and dissolved oxygen using a membrane made from polymerizable sensing monomers. Sensors Actuators B Chem 2010–06 147(2):714–722
Tou ZQ, Chan CC, Hong J, Png S, Eddie KMT, Tan TAH (2014) Double-pass Mach–Zehnder fiber interferometer pH sensor. J Biomed Opt 19(4):047002
Vander Heiden MG, Cantley LC, Thompson CB (2009) Understanding the Warburg effect: the metabolic requirements of cell proliferation. J Sci 324(5930):1029–1033
Funding
This study was supported by the National Natural Science Foundation of China (NSFC) (No. 61873168).
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This article is part of the topical collection on Role of Nanotechnology and Internet of Things in Healthcare
This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s11051-021-05231-3
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Hu, Y., Zheng, C., Tao, W. et al. RETRACTED ARTICLE: Fluorescence spectrum–based dual-parameter detection method for pH and DO in cancer cell metabolic fluid. J Nanopart Res 22, 186 (2020). https://doi.org/10.1007/s11051-020-04900-z
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DOI: https://doi.org/10.1007/s11051-020-04900-z