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Estimation of movement from insectary plants to crop plants in Orius bugs (Heteroptera: Anthocoridae) by molecular gut content analysis

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Abstract

The introduction of insectary and banker plants is widely used in agroecosystems to improve the survival and reproduction of natural enemies of pest arthropods. Natural enemies that are attracted to and/or maintained on insectary plants need to move from the insectary plants to crop plants to suppress pest populations effectively. In many cases, however, direct evidence supporting the migration of natural enemies is lacking. To investigate the movement of Orius bugs (Hemiptera: Anthocoridae), which are efficient zoophytophagous predators used in biological control, we performed gut content analyses based on DNA markers. Specific PCR primers were designed to detect the DNA of the insectary plant, Scaevola aemula. We also carried out gut content analyses using prey insect DNA markers to estimate Orius bugs’ predation spectrum. DNA of the insectary plant was detected in the abdomen of Orius bugs collected on eggplant leaves, suggesting that Orius bugs had consumed food resources from the insectary plants and relocated to the crop plants. DNA of three prey insect species—two thrips species, Thrips palmi and Frankliniella intonsa, and cotton aphid, Aphis gossypii—was detected in Orius bugs in the eggplant field, indicating that Orius bugs preyed on them.

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References

  • Agustí N, Castañé C, Fraile I, Alomar O (2020) Development of a PCR-based method to monitor arthropod dispersal in agroecosystems: Macrolophus pygmaeus (Hemiptera: Miridae) from banker plants to tomato crops. Insect Sci. https://doi.org/10.1111/1744-7917.12717

    Article  Google Scholar 

  • Armer CA, Wiedenmann RN, Bush DR (1998) Plant feeding site selection on soybean by the facultatively phytophagous predator Orius insidiosus. Entomol Exp Appl 86:109–118

    Article  Google Scholar 

  • Begum M, Gurr GM, Wratten SD, Hedberg PR, Nicol HI (2006) Using selective food plants to maximize biological control of vineyard pests. J Appl Ecol 43:547–554

    Article  Google Scholar 

  • Berndt LA, Wratten SD (2005) Effects of alyssum flowers on the longevity, fecundity, and sex ratio of the leafroller parasitoid Dolichogenidea tasmanica. Biol Control 32:65–69

    Article  Google Scholar 

  • Colley MR, Luna JM (2000) Relative attractiveness of potential beneficial insectary plants to aphidophagous hoverflies (Diptera: Syrphidae). Environ Entomol 29:1054–1059

    Article  Google Scholar 

  • Gomez-Polo P, Alomar O, Castañé C, Aznar-Fernández T, Lundgren JG, Piñol J, Agustí N (2016) Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods. Pest Manag Sci 72:272–279

    Article  CAS  Google Scholar 

  • Gontijo LM, Beers EH, Snyder WE (2013) Flowers promote aphid suppression in apple orchards. Biol Control 66:8–15

    Article  Google Scholar 

  • González-Chang M, Tiwari S, Sharma S, Wratten SD (2019) Habitat management for pest management: limitations and prospects. Ann Entomol Soc Am 112:302–317

    Article  Google Scholar 

  • Harper GL, King RA, Dodd CS, Harwood JD, Glen DM, Bruford MW, Symondson WOC (2005) Rapid screening of invertebrate predators for multiple prey DNA targets. Mol Ecol 14:819–827

    Article  CAS  Google Scholar 

  • Hinomoto N, Muraji M, Noda T, Shimizu T, Kawasaki K (2004) Identification of five Orius species in Japan by multiplex polymerase chain reaction. Biol Control 31:276–279

    Article  CAS  Google Scholar 

  • Hogg BN, Nelson EH, Mills NJ, Daane KM (2011) Floral resources enhance aphid suppression by a hoverfly. Entomol Exp Appl 141:138–144

    Article  Google Scholar 

  • Itou M, Watanabe M, Watanabe E, Miura K (2013) Gut content analysis to study predatory efficacy of Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae) by molecular methods. Entomol Sci 16:145–150

    Article  Google Scholar 

  • King RA, Read DS, Traugott M, Symondson WOC (2008) Molecular analysis of predation: a review of best practice for DNA-based approaches. Mol Ecol 17:947–963

    Article  CAS  Google Scholar 

  • Lattin JD (1999) Bionomics of the Anthocoridae. Ann Rev Entomol 44:207–231

    Article  CAS  Google Scholar 

  • Nagamori S, Hikawa M, Nagashima S, Kondo A, Sano T, Nagai K, Nakasuji F (2010) Screening of ground-cover plants for conservation of a predacious bugs, Orius spp., in a biological control program for vegetables. Bull Okayama Pref Agri 1:5–12 (in Japanese with English summary)

    Google Scholar 

  • Ohta I, Takeda M (2014) Adult survival of Orius strigicollis (Poppius) on different flowering plants and its development and fecundity on buckwheat flowers. Ann Rept Kansai Pl Prot 56:1–5 (in Japanese with English abstract)

    Article  Google Scholar 

  • Parolin P, Bresch C, Poncet C, Desneux N (2012) Functional characteristics of secondary plants for increased pest management. Int J Pest Manag 58:369–377

    Article  Google Scholar 

  • Pumariño L, Alomar O (2012) The role of omnivory in the conservation of predators: Orius majusculus (Heteroptera: Anthocoridae) on sweet alyssum. Biol Control 62:24–28

    Article  Google Scholar 

  • Ribeiro AL, Gontijo LM (2017) Alyssum flowers promote biological control of collard pests. Biocontrol 62:185–196

    Article  Google Scholar 

  • Riudavets J, Castañé C (1998) Identification and evaluation of native predators of Frankliniella occidentalis (Thysanoptera: Thripidae) in the Mediterranean. Environ Entomol 27:86–93

    Article  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Method Mol Biol 132:365–386

    CAS  Google Scholar 

  • Seko T, Abe J, Miura K, Hikawa M (2017) The contribution of a beneficial insectary plant Scaevola aemula to survival and long-term establishment of flightless Harmonia axyridis in greenhouses. Biocontrol 62:221–231

    Article  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  Google Scholar 

  • Waite MO, Scott-Dupree CD, Brownbridge M, Buitenhuis R, Murphy G (2014) Evaluation of seven plant species/cultivars for their suitability as banker plants for Orius insidiosus (Say). Biocontrol 59:79–87

    Article  CAS  Google Scholar 

  • Wang Q, Bao WF, Yang F, Xu B, Yang YZ (2017) The specific host plant DNA detection suggests a potential migration of Apolygus lucorum from cotton to mungbean fields. PLoS ONE 12:e0177789

    Article  CAS  Google Scholar 

  • Xu X, Borgemeister C, Poehling HM (2006) Interactions in the biological control of western flower thrips Frankliniella occidentalis (Pergande) and two-spotted spider mite Tetranychus urticae Koch by the predatory bug Orius insidiosus Say on beans. Biol Control 36:57–64

    Article  CAS  Google Scholar 

  • Yano E (2004) Recent development of biological control and IPM in greenhouses in Japan. J Asia Pac Entomol 7:5–11

    Article  Google Scholar 

  • Yasunaga T (1997a) The flower bug genus Orius Wolff (Heteroptera: Anthocoridae) from Japan and Taiwan, part I. Appl Entomol Zool 32:355–364

    Article  Google Scholar 

  • Yasunaga T (1997b) The flower bug genus Orius Wolff (Heteroptera: Anthocoridae) from Japan and Taiwan, Part II. Appl Entomol Zool 32:379–386

    Article  Google Scholar 

  • Yasunaga T (1997c) The flower bug genus Orius Wolff (Heteroptera: Anthocoridae) from Japan and Taiwan, Part III. Appl Entomol Zool 32:387–394

    Article  Google Scholar 

  • Zhao J, Guo X, Tan X, Desneux N, Zappala L, Zhang F, Wang S (2017) Using Calendula officinalis as a floral resource to enhance aphid and thrips suppression by the flower bug Orius sauteri (Hemiptera: Anthocoridae). Pest Manag Sci 73:515–520

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. National Agriculture and Food Research Organization Western Region Agricultural Research Center, Nishifukatsu 6-12-1, Fukuyama, Hiroshima, 721-8514, Japan

    Masayuki Hayashi, Junichiro Abe, Yuta Owashi & Kazuki Miura

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  1. Masayuki Hayashi
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  2. Junichiro Abe
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  3. Yuta Owashi
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  4. Kazuki Miura
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Correspondence to Kazuki Miura.

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Hayashi, M., Abe, J., Owashi, Y. et al. Estimation of movement from insectary plants to crop plants in Orius bugs (Heteroptera: Anthocoridae) by molecular gut content analysis. Appl Entomol Zool 55, 361–365 (2020). https://doi.org/10.1007/s13355-020-00692-9

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