Skip to main content
SpringerLink
Log in

Analysis of the Receptor Specificity of Influenza A Virus Strains Using the Method of Surface Plasmon Resonance

  • MOLECULAR BIOPHYSICS
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract—The receptor specificity for the A/rook/Chany/32/2015 (H5N1), A/common gull/Saratov/1676/2018 (H5N6), and A/chicken/PrimorskyKrai/03/2018 (Н9N2) strains of avian influenza virus has been studied. Using the method of surface plasmon resonance for the selected strains we obtained the values ​​of the equilibrium dissociation constants with analogues of receptors such as α2-3 and α2-6. It was found that for strains A/rook/Chany/32/2015 (H5N1) and A/common gull/Saratov/1676/2018 (H5N6), a binding with the human type of receptor (α2-6) is determined, in contrast to the strain A/chicken/PrimorskyKrai/03/2018 (H9N2), which is specific only for avian type receptors (α2-3).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. T.N. Il’icheva, S. V. Netesov, and V. N. Gureev, Infuenza Viruses: Methodological Manual (Novosibirsk State Univ., Novosibirsk, 2014), Part 1.

  2. A. S. Gambaryan, S. S. Iamnikova, D. K. L’vov, et al., Mol. Biol. (Moscow) 36, 542 (2002).

    Article  Google Scholar 

  3. K. Shinya, M. Ebina, S. Yamada, et al., Nature 440, 435 (2006).

    Article  ADS  Google Scholar 

  4. G. S. Onkhonova, S. V. Mal’tsev, and A. B. Ryzhikov, Biophysics (Moscow) 64 (4), 522 (2019).

    Article  Google Scholar 

  5. Virology: A Practical Approach, Ed. by B. W. J. Mahy (IRL, Oxford, 1985; Mir, Moscow, 1988).

  6. R. K. Scopes, Protein Purification: Principles and Practice (Springer, New York, 1982; Mir, Moscow, 1985).

Download references

Funding

This study was carried out as part of State Assignment no. 13/19 Development of genetic and phenotypic markers for the quantitative assessment of pandemic potential of zoonotic influenza viruses.

Author information

Authors and Affiliations

  1. The VECTOR State Research Center of Virology and Biotechnology, 630559, Koltsovo, Novosibirsk oblast, Russia

    G. S. Onkhonova, P. Yu. Torzhkova, V. Yu. Marchenko, S. V. Svyatchenko, A. S. Gudymo & A. B. Ryzhikov

Authors
  1. G. S. Onkhonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Yu. Torzhkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Yu. Marchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. V. Svyatchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. S. Gudymo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. B. Ryzhikov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. S. Onkhonova.

Ethics declarations

In carrying out this work, all ethical standards were observed.

Conflict of Interest. The authors declare that they have no conflicts of interest.

This work does not contain a description of any studies using humans and animals as objects.

Additional information

Translated by A. Ostyak

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Onkhonova, G.S., Torzhkova, P.Y., Marchenko, V.Y. et al. Analysis of the Receptor Specificity of Influenza A Virus Strains Using the Method of Surface Plasmon Resonance . BIOPHYSICS 65, 222–224 (2020). https://doi.org/10.1134/S0006350920020153

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350920020153

Search

Navigation