Abstract
The humpback grouper (Cromileptes altivelis) is a commercially important species of the family Epinephelidae. With the development in aquaculture industry, C. altivelis breeding has gradually increased in volumetric production, leading to the occurrence of various diseases. In this study, we established a new cell line (CAM) derived from the muscle tissue of C. altivelis. Our results showed that the optimal growth temperature and working concentration of fetal bovine serum (FBS) of CAM cells were 28 °C and 15%, respectively. DNA sequencing and comparative analysis of 18S rRNA gene sequence showed that CAM cell line was originated from C. altivelis. Chromosome analysis showed that the modal chromosome number of CAM cells was 48. After transfection using pEGFP-N3 plasmid, CAM cells exhibited high transfection efficiency, indicating that CAM cells could be used in foreign gene expression studies. Further, cytotoxicity analysis revealed that CAM cells were sensitive to Vibrio harveyi and Edwardsiella tarda. Moreover, the cytotoxicity of heavy metals (Hg, Cd, and Cu) to CAM cells was dose-dependent. This CAM cell line might be used as an ideal tool in vitro for analyzing and understanding the mechanisms of pathogenesis, host-pathogen interactions, and toxicity assay of heavy metals.
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Funding
This research was supported financially by the Natural Science Foundation of Hainan Province (No. 2019RC078), Key Research Project of Hainan Province (ZDKJ2019011), and Marine Economic, Innovative Demonstration City Project of State Oceanic Administration (HHCL201813, HHCL201802), and the Nanhai Famous Youth Project.
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Animal care was performed conformity with NIH guidelines (NIH Pub. No. 85e23, revised 1996) and was approved by Animal Care and Use Committee of the Hainan University.
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Wang, L., Cao, Z., Liu, Y. et al. Establishment and characterization of a new cell line from the muscle of humpback grouper (Cromileptes altivelis). Fish Physiol Biochem 46, 1897–1907 (2020). https://doi.org/10.1007/s10695-020-00841-5
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DOI: https://doi.org/10.1007/s10695-020-00841-5