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Complete genome analysis of Pantoea agglomerans-infecting bacteriophage vB_PagM_AAM22

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Abstract

A novel myovirus, vB_PagM_AAM22 (AAM22), was isolated in Lithuania using Pantoea agglomerans as the host for phage propagation. The 49,744-bp genome of AAM22 has a G + C content of 48.4% and contains 96 probable protein-encoding genes and no genes for tRNA. In total, 34 ORFs were given a putative functional annotation, including genes associated with virion morphogenesis, DNA metabolism, and phage-host interactions. Based on comparative phylogenetic analysis, AAM22 cannot be assigned to any genus currently recognized by the ICTV and is a potential candidate to form a new genus within the family Myoviridae.

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Acknowledgements

This research was funded by Grants (no. 09.3.3.-LMT-K-712-15-0109 and no. 09.3.3.-LMT-K-712-16-0087) from the Research Council of Lithuania.

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Authors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av. 7, 10257, Vilnius, Lithuania

    Lidija Truncaitė, Monika Šimoliūnienė, Laurynas Alijošius, Emilija Petrauskaitė, Laura Kiaušaitė, Rolandas Meškys & Eugenijus Šimoliūnas

  2. Center for Physical Sciences and Technology, Saulėtekio av. 3, Vilnius, Lithuania

    Martynas Skapas

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  1. Lidija Truncaitė
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  2. Monika Šimoliūnienė
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  8. Eugenijus Šimoliūnas
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Correspondence to Eugenijus Šimoliūnas.

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Truncaitė, L., Šimoliūnienė, M., Alijošius, L. et al. Complete genome analysis of Pantoea agglomerans-infecting bacteriophage vB_PagM_AAM22. Arch Virol 165, 2111–2114 (2020). https://doi.org/10.1007/s00705-020-04705-4

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