Abstract
Trail-following behavior is a key to ecological success of termites, allowing them to orient themselves between the nesting and foraging sites. This behavior is controlled by specific trail-following pheromones produced by the abdominal sternal gland occurring in all termite species and developmental stages. Trail-following communication has been studied in a broad spectrum of species, but the “higher” termites (i.e. Termitidae) from the subfamily Syntermitinae remain surprisingly neglected. To fill this gap, we studied the trail-following pheromone in six genera and nine species of Syntermitinae. Our chemical and behavioral experiments showed that (3Z,6Z,8E)-dodeca-3,6,8-trien-1-ol is the single component of the pheromone of all the termite species studied, except for Silvestritermes euamignathus. This species produces both (3Z,6Z)-dodeca-3,6-dien-1-ol and neocembrene, but only (3Z,6Z)-dodeca-3,6-dien-1-ol elicits trail-following behavior. Our results indicate the importance of (3Z,6Z,8E)-dodeca-3,6,8-trien-1-ol, the most widespread communication compound in termites, but also the repeated switches to other common pheromones as exemplified by S. euamignathus.
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Acknowledgements
We thank Divino Brandão, Pedro Henrique Ferri and Neuricio Ricardo Azevedo from Universidade Federal de Goiás, Goiânia, state of Goiás, Brazil (Goiania), Luiz Carlos Forti, from Universidade Estadual de São Paulo, Botucatu, State of São Paulo, Brazil, for providing laboratory facilities during our field studies, Karine Gourrat, from Centre des Sciences du Goût et de l’Alimentation, Dijon, France for LRI measurements of standards, and Adrienne Gastineau for technical assistance. We are grateful to the staff of the Laboratoire Environnement HYDRECO of Petit Saut (Guyane) for logistic support. JŠ and TB were supported by project of Internal Grant Agency of Faculty of Tropical AgriSciences number 20195006. EMC thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the grant (Proc. 3076681/2016-5\0).
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The study was designed by DSD, AR and CB. Material preparation, data collection and analysis were performed by DSD, JŠ, TB, PW, ES, EMC, CL and CB. The first draft of the manuscript was written by DSD, JŠ and CB and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Sillam-Dussès, D., Šobotník, J., Bourguignon, T. et al. Trail-Following Pheromones in the Termite Subfamily Syntermitinae (Blattodea, Termitoidae, Termitidae). J Chem Ecol 46, 475–482 (2020). https://doi.org/10.1007/s10886-020-01180-8
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DOI: https://doi.org/10.1007/s10886-020-01180-8