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Intestinal Microbiota Analyses of Litopenaeus vannamei During a Case of Atypical Massive Mortality in Northwestern Mexico

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Abstract

This study investigated the intestinal microbial community structure of Litopenaeus vannamei at six different stages during shrimp farming. Our goal was to elucidate the bacterial profile and the changes in the relative abundance of taxa during an atypical massive mortality event in Sonora, Mexico. High-throughput sequencing of the 16S rRNA gene and denaturing gradient gel electrophoresis showed that Vibrionaceae was persistent with high relative abundances in the intestine from cultivated shrimp during all the studied stages. The massive mortality observed at day 63 could be related to an overabundance of different Operational Taxonomic Units (OTUs) of Vibrio, Shewanella and Clostridium. Principal coordinate analysis (PCoA) showed variations in microbial structure at different culture times. These findings suggest that OTUs of different taxa contributed to the community switch from healthy to diseased individuals, questioning the hypothesis that single bacterial species is the cause of disease outbreaks. This study provided data to improve the understanding of disease outbreaks during shrimp farming.

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Acknowledgements

This project was supported by Consejo Nacional de Ciencia y Tecnología, Grants 175268 (FINNOVA 2012–2014) and 178664 (PROINNOVA 2012–2013) to ALC.

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Authors and Affiliations

  1. Laboratorio de Geomicrobiología y Biotecnología, Centro de Investigaciones Biológicas del Noroeste, La Paz, BCS, México

    Alejandro López-Cortés & Hever Latisnere-Barragán

  2. CONACYT - Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Unidad Mérida, Yucatán, México

    José Q. García-Maldonado

  3. Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada Baja California, CICESE, 22860, Ensenada, BC, Mexico

    Miguel A. Martínez

  4. Conservation Genetics Laboratory & Desert Laboratory on Tumamoc Hill, University of Arizona, Tucson, AZ, 85721, USA

    Adrián Munguía-Vega

  5. @ Lab Applied Genomics Research, 23000, La Paz, BCS, Mexico

    Adrián Munguía-Vega

  6. Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Colonia Playa Palo de Santa Rita Sur, 23096, La Paz, BCS, México

    Alejandro López-Cortés

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  1. Alejandro López-Cortés
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  5. Adrián Munguía-Vega
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Contributions

The corresponding author performed the study conception and design. Material preparation, data collection and analysis were performed by JQG-M, AM-V, MM and HL-B. The first draft of the manuscript was written by AL-C and all authors participated in drafting the sections of the manuscript and commented on previous versions.

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Correspondence to Alejandro López-Cortés.

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The specimens are aquaculture animals. The animals were handled under the best practices and for sampling, the principles of Replacement, Reduction and Refinement (3Rs) were applied.

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López-Cortés, A., Latisnere-Barragán, H., García-Maldonado, J.Q. et al. Intestinal Microbiota Analyses of Litopenaeus vannamei During a Case of Atypical Massive Mortality in Northwestern Mexico. Curr Microbiol 77, 2312–2321 (2020). https://doi.org/10.1007/s00284-020-02079-z

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