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Enhancement of S-adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens

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Abstract

Objectives

To improve the S-adenosylmethionine (SAM) production in methionine-free medium, effects of deleting genes of SAM decarboxylase (speD) and homoserine kinase (thrB) on SAM titers were investigated, and the SAM synthetase gene (SAM2) was also overexpressed.

Results

In B. amyloliquefaciens HSAM2, deleting speD to block the SAM utilization pathway significantly reduced the SAM titer. After knockout of thrB to block the branched pathway, the resulted mutant HSAM4 produced 143.93 mg/L SAM, increasing by 42% than HSAM2. Further plasmid-based expression of SAM2 improved the SAM titer to 226.92 mg/L, and final optimization of key fermentation parameters resulted in the maximum SAM titer of 412.01 mg/L in flasks batch fermentation.

Conclusions

Deleting thrB and overexpressing SAM2 gene were efficient for enhanced SAM production in B. amyloliquefaciens. The maximum SAM titer in flasks batch fermentation was much higher than that of previous reports.

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Supporting information

Supplementary Table 1—Primers used for PCR in this study.

Funding

This work was supported by Da Bei Nong Group Promoted Project for Young Scholar of HZAU (No. 2017DBN011) and the National Natural Science Foundation of China (31501468).

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Authors and Affiliations

  1. Key Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Cong Jiang, Liying Ruan, Xuetuan Wei & Ailing Guo

Authors
  1. Cong Jiang
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  2. Liying Ruan
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  3. Xuetuan Wei
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  4. Ailing Guo
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Correspondence to Xuetuan Wei or Ailing Guo.

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Jiang, C., Ruan, L., Wei, X. et al. Enhancement of S-adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens. Biotechnol Lett 42, 2293–2298 (2020). https://doi.org/10.1007/s10529-020-02945-7

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  • DOI: https://doi.org/10.1007/s10529-020-02945-7

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