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Phosphorylation of Tyrosine and Tyr-Thr-Lys Tripeptide with Cyclohexylmethyl- and (Deuteromethyl)phosphonochloridates

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Abstract

Methods for phosphorylation of tyrosine and Tyr-Thr-Lys tripeptide with cyclohexylmethyl(deuteromethyl)phosphonochloridates have been developed. These products can be used as reference compounds in the analysis of blood plasma of patients presumably exposed to cyclohexylmethylphosphonofluoridate (cyclosarin) action. Conditions for the separation and purification of the synthesized intermediates by means of chromatography have been determined and optimized, allowing high-purity phosphorylated products at the final stage of the synthesis.

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Funding

This study was financially supported by the Russian Science Foundation (project no. 19-13-00057).

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Authors and Affiliations

  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    I. A. Rodin & T. M. Baygildiev

  2. Laboratory for the Chemical and Analytical Control of the Military Research Center, 105005, Moscow, Russia

    V. I. Krylov, I. I. Krylov, V. A. Yashkir & I. V. Rybalchenko

  3. Pharm-Sintez, 111024, Moscow, Russia

    V. N. Osipov

Authors
  1. I. A. Rodin
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  2. T. M. Baygildiev
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  3. V. I. Krylov
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  4. V. N. Osipov
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  5. I. I. Krylov
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  6. V. A. Yashkir
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  7. I. V. Rybalchenko
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Correspondence to I. A. Rodin.

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Rodin, I.A., Baygildiev, T.M., Krylov, V.I. et al. Phosphorylation of Tyrosine and Tyr-Thr-Lys Tripeptide with Cyclohexylmethyl- and (Deuteromethyl)phosphonochloridates. Russ J Gen Chem 90, 619–623 (2020). https://doi.org/10.1134/S1070363220040106

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  • DOI: https://doi.org/10.1134/S1070363220040106

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