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First molecular characterization of avian paramyxovirus-1 (Newcastle disease virus) in Botswana

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Abstract

Avian paramyxovirus-1 (APMV-1), the causative agent of Newcastle disease (ND) in domestic and wild avian species, has recently been reported and characterized in five southern African countries (i.e. Mozambique, Namibia, South Africa, Zambia and Zimbabwe). Since APMV-1s have never been characterized in Botswana, this study was undertaken to determine the genotype circulating in the country. Fourteen samples were collected from ND outbreaks in poultry in 2014, 2018 and 2019 and the complete fusion protein gene was sequenced. Phylogenetic analysis revealed that all of the viruses from Botswana clustered in genotype VII.2 (previously subgenotype VIIh) and that they were more related to viruses from South Africa and Mozambique than the other southern African countries (i.e. Namibia, Zambia and Zimbabwe).

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Acknowledgments

The sequences from this study were generated through the Sequencing Services of the Animal Production and Health sub-programme of the Joint Food and Agricultural Organization of the United Nations/International Atomic Energy Agency (IAEA) Division. This study was supported by funds from the IAEA Peaceful Uses Initiative (PUI) VETLAB Network.

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Authors and Affiliations

  1. Botswana National Veterinary Laboratory, Private Bag 0035, Gaborone, Botswana

    Tebogo Kgotlele, Boitumelo Modise, John Fredrick Nyange & Carter Thanda

  2. Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria

    Giovanni Cattoli & William G. Dundon

  3. Animal Production and Health Laboratory, IAEA, Freidenstrasse, 1, 2444, Seibersdorf, Austria

    William G. Dundon

Authors
  1. Tebogo Kgotlele
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  2. Boitumelo Modise
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  3. John Fredrick Nyange
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  4. Carter Thanda
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  5. Giovanni Cattoli
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  6. William G. Dundon
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Contributions

TK, BM, GC, WGD: conceived and designed the study. TK, JFN, CT: collected and analysed the samples. TK, BM, WGD: analysed the sequence data. WGD: wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to William G. Dundon.

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The authors declare no conflicts of interest.

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This article does not contain studies with animals performed by any of the authors.

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Edited by Detlev H. Kruger.

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Kgotlele, T., Modise, B., Nyange, J.F. et al. First molecular characterization of avian paramyxovirus-1 (Newcastle disease virus) in Botswana. Virus Genes 56, 646–650 (2020). https://doi.org/10.1007/s11262-020-01770-4

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  • DOI: https://doi.org/10.1007/s11262-020-01770-4

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