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Effects of the PI3K/Akt signaling pathway on the apoptosis of early host cells infected with Eimeria tenella

  • Immunology and Host-Parasite Interactions - Original Paper
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Abstract

This study investigated the role of PI3K/Akt signaling pathway on host cell apoptosis in the early infection of Eimeria tenella. Chicken cecal epithelial cells were treated with apoptosis-inducer Actinomycin D (Act D) or PI3K/Akt signaling pathway inhibitor LY294002 and then infected with E. tenella. Results demonstrated that the E. tenella-infected group had less apoptosis 4–8 h after the infection and more apoptosis 12–20 h after the infection than the control group. At 4–20 h after the infection, the apoptotic/necrotic rate and the Caspase-3 activity in the Act D + E. tenella group were lower (P < 0.01) than those in the Act D-treated group. The p-Akt and NF-κB contents in the E. tenella-infected group were higher (P < 0.01) than those in the control group 4–12 h after the infection. However, the bad content and the Caspase-9/3 activity were lower (P < 0.05) in the E. tenella-infected group than in the control group. Compared with the E. tenella-infected group, the LY294002 + E. tenella group showed decreased p-Akt content and increased apoptotic/necrotic rate, bad content, NF-κB expression, membrane permeability transition pore (MPTP) openness, and Caspase-9/3 activity. Thus, the early development of E. tenella could inhibit host cell apoptosis by downregulating the Caspase-3 activity. Upregulating this activity promoted apoptosis. In addition, activating the PI3K/Akt signaling pathway inhibited the apoptosis of E. tenella host cells in the early infection by reducing the expression of the bad content, limiting the MPTP opening, and decreasing the Caspase-9 and Caspase-3 activities.

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Funding

This study was funded by a grant from the National Natural Science Foundation of China (Grant No. 31972647).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, China

    Xuesong Zhang, Shan Li, Mingxue Zheng, Li Zhang, Rui Bai, Ruiqi Li, Siyuan Hao, Bing Bai & Huixin Kang

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  1. Xuesong Zhang
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  2. Shan Li
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  3. Mingxue Zheng
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  4. Li Zhang
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  8. Bing Bai
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  9. Huixin Kang
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Correspondence to Mingxue Zheng.

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All work involving animals was conducted in accordance with the national legislation on the protection of animal welfare and following the strict guidelines and approval of the Institution Animal Care and Use Committee of Shanxi Agricultural University, China.

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Section Editor: Berit Bangoura

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figure 12

The group of E. tenella (up), the group of E. tenella + Act D (low) at 4–20 h after the infection, respectively, Sporozoites are indicated by arrows (H&E staining)

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Zhang, X., Li, S., Zheng, M. et al. Effects of the PI3K/Akt signaling pathway on the apoptosis of early host cells infected with Eimeria tenella. Parasitol Res 119, 2549–2561 (2020). https://doi.org/10.1007/s00436-020-06738-9

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