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Extracts of the Brown Alga Dictyota dichotoma (Hudson) J.V. Lamouroux Alleviate Salt Stress in Rice (Oryza sativa L.) During Germination

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Abstract

Algal extracts provide a safe regime for enhancing crop productivity under stressful conditions. The present study evaluated the efficiency of aqueous and ethanolic extracts of the brown alga Dictyota dichotoma in alleviation of salt stress on germination of rice seeds. Firstly, seeds were germinated using the aqueous extract of D. dichotoma at concentrations of 0, 5, 10, 20, and 50 g L−1, prepared either at room temperature (RTE) or by boiling (BLE). The % germination of rice increased from 84% in non-treated seeds to 100% when treated with 20 g L−1 BLE, although this treatment caused reduced uniformity of germination. Embryo growth was maximum at 20 g L−1 of both extracts with superiority of BLE over RTE. In the second experiment, the effect of 20 g L−1 aqueous and ethanolic extracts relative to a balanced nutrient supply on germination of seeds treated with 0, 40, 90, and 170 mM NaCl was investigated. Salinity reduced % germination with delayed onset but high uniformity of germination, whereas algal amendments counterbalanced the effect of salinity to a greater extent relative to the nutrient supply. Upon withdrawal of salt stress, seeds promptly recovered, with more efficient recovery of seeds exposed to 170 mM than from 90 mM NaCl. The lower recovery of salt-treated seeds compared with the control seed germination suggests that rice suffered from the toxic ion effect of salinity on embryo rather than from the osmotic effect. Extracts of D. dichotoma can enhance and also alleviate salinity stress on rice seed germination.

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Acknowledgements

The authors express their deep thanks to the Experimental Station of Agricultural Research at Giza, Egypt for providing the seeds of the experimental rice cultivar.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta City, 34517, Egypt

    Taha Mohamed El-Katony, Mohamed Ali Deyab, Magda Faiz El-Adl & Fatma Mohamed El-Nabway Ward

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  1. Taha Mohamed El-Katony
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  2. Mohamed Ali Deyab
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  3. Magda Faiz El-Adl
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  4. Fatma Mohamed El-Nabway Ward
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Correspondence to Taha Mohamed El-Katony.

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El-Katony, T.M., Deyab, M.A., El-Adl, M.F. et al. Extracts of the Brown Alga Dictyota dichotoma (Hudson) J.V. Lamouroux Alleviate Salt Stress in Rice (Oryza sativa L.) During Germination. J Plant Growth Regul 40, 986–999 (2021). https://doi.org/10.1007/s00344-020-10156-7

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