Skip to main content
SpringerLink
Log in

Detection of QTL and QTL × environment interaction for scald resistance in a two-row × six-row cross of barley

  • Original Paper
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

Leaf scald caused by Rhynchosporium secalis, is a serious disease of barley (Hordeum vulgare L.) in Tunisia. A doubled-haploid barley population, from a cross between the two-rowed ‘Roho’ and the six-rowed ‘L90’ cultivars, was evaluated for its reaction to scald under natural infection in replicated field trials for two years. Phenotypic variation among doubled-haploid lines was significant for disease reaction in both environments (cropping seasons) and genotype × environment interaction was also significant. Heritability estimates were high in both seasons and significant correlation was noted between scald reactions recorded in the two seasons. Segregation of 34 single sequence repeat markers was used for quantitative trait loci mapping. Single-marker analysis was applied for individual and for combined environments to determine the effect of each molecular marker on scald resistance. In total, six QTLs with additive effects were mapped on chromosomes 2H, 3H, 4H, 6H and 7H which explained from 4.4 to 11.7% of the phenotypic variance. Among these, only QTLs linked to WMS6 (4H) and to HVM31 (6H) showed significant QTL × environment interactions. QTLs associated to HVM36 (2H) and to Bmag0571 (7H) were the most significant for scald resistance and the most insensitive to the environment. Another stable QTL was identified, for the first time, in the vicinity of the Vrs1 locus on the 2H chromosome. For this QTL, the two-rowed parental allele reduced disease severity. These chromosomal regions that affect scald resistance independently of the environment would be of great interest in marker-assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bjørnstad A, Grønnerød S, Mac Key J, Tekauz A, Crossa J, Martens H (2004) Resistance to barley scald (Rhynchosporium secalis) in the Ethiopian donor lines ‘Steudelli’ and ‘Jet’, analyzed by partial least squares regression and interval mapping. Hereditas 141:166–179

    Article  Google Scholar 

  • Bouajila A, Abang MM, Haouas S, Udupa S, Rezgui S, Baum M, Yahyaoui AH (2007) Genetic diversity of Rhynchosporium secalis in Tunisia as revealed by pathotype, AFLP and microsatellite analyses. Mycopathologia 163:281–294

    Article  Google Scholar 

  • Cakir M, Wallwork H, Gupta S, Moody D, Williams K, Li CD (2004) Identification of new adult plant resistance gene for scald (Rhynchosporium secalis) in barley. In: Spunar J, Janikova J (eds) Proceedings of the 9th international barley genetics symposium (IBGS 2004). Kromeriz, Czech Republic, pp 644–648

  • Cheong J, Williams K, Wallwork H (2006) The identification of QTLs for adult plant resistance to leaf scald in barley. Aust J Agric Res 57:961–965

    Article  CAS  Google Scholar 

  • Cherif M, Rezgui S, Devaux P, Harrabi M (2008) Genetic analysis of net blotch resistance in a two-row × six-row cross of barley, using doubled-haploid lines. Can J Plant Sci 88:257–266

    Article  Google Scholar 

  • Choo TM, Vigier B, Shen QQ, Martin RA, Ho KM, Savard M (2004) Barley traits associated with resistance to fusarium heat blight and deoxynivalenol accumulation. Phytopathology 94:1145–1150

    Article  CAS  Google Scholar 

  • Frégeau-Reid J, Choo TM, Ho KM, Martin RA, Konishi T (2001) Comparisons of two-row and six-row barley for chemical composition using doubled-haploid lines. Crop Sci 41:1737–1743

    Article  Google Scholar 

  • Genger RK, Nesbitt K, Brown AHD, Abbott DC, Burdon JJ (2005) A novel barley scald resistance gene: genetic mapping of the Rrs 15 scald resistance gene derived from wild barley Hordeum vulgare ssp spontaneum. Plant Breed 124:137–141

    Article  CAS  Google Scholar 

  • Hanson S (2016) Tunisia grain and feed annual. Gain report. Global Agricultural Information Network. USDA Foreign Agricultural Service

  • Hofmann K (2014) Phenotypic assessment and genetic mapping of genes conferring resistance to leaf scald (Rhynchosporium commune) in barley (Hordeum vulgare). Ph.D. in Agricultural Sciences. Justus Liebig Giessen University, Germany

  • Jansen RC, Van Ooijen JW, Stam P, Lister C, Dean C (1995) Genotype-by-environment interaction in genetic mapping of multiple quantitative trait loci. Theor Appl Genet 91:33–37

    Article  CAS  Google Scholar 

  • Li CD, Lance CM, Collins HM, Tarr A, Roumeliotis S, Harasymow S, Cakir M, Fox GP, Grime CR, Broughton S, Young KJ, Raman H, Barr AR, Moody DB, Read BJ (2003) Quantitative trait loci controlling kernel discoloration in barley (Hordeum vulgare L.). Aust J Agric Res 54:1251–1259

    Article  CAS  Google Scholar 

  • Li YB, Wu CJ, Jiang GH, Wang LQ, He YQ (2007) Dynamic analyses of rice blast resistance for the assessment of genetic and environmental effects. Plant Breed 126:541–547

    Article  Google Scholar 

  • Liu H, Bayer M, Druka A, Russell JR, Hackett CA, Poland J, Ramsay L, Hedley PE, Waugh R (2014) An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley. BMC Genomics 15:104. https://doi.org/10.1186/1471-2164-15-104

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Looseley ME, Newton AC, Atkins SD, Fitt BDL, Fraaije BA, Thomas WTB, Keith R, Macaulay M, Lynott J, Harrap D (2012) Genetic basis of control of Rhynchosporium secalis infection and symptom expression in barley. Euphytica 184:47–56

    Article  Google Scholar 

  • Looseley ME, Keith R, Guy D, Barral-Baron G, Thirugnanasambandam A, Harrap D, Werner P, Newton AC (2015) Genetic mapping of resistance to Rhynchosporium commune and characterisation of early infection in a winter barley mapping population. Euphytica 203:337–347

    Article  CAS  Google Scholar 

  • Pillen K, Binder A, Kreuzkam B, Ramsay L, Waugh R, Förster J, Léon J (2000) Mapping new EMBL-derived barley microsatellites and their use to differentiate German barley cultivars. Theor Appl Genet 101:652–660

    Article  CAS  Google Scholar 

  • Pillen K, Zacharias A, Leon J (2003) Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet 107:340–352

    Article  CAS  Google Scholar 

  • Ramsay L, Macaulay M, Degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    CAS  PubMed  PubMed Central  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA sepacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8019

    Article  CAS  Google Scholar 

  • Sameri M, Takeda K, Komatsuda T (2006) Quantitative trait loci controlling agronomic traits in recombinant inbred lines from a cross of oriental- and occidental-type barley cultivars. Breed Sci 56:243–252

    Article  Google Scholar 

  • SAS Institute, Inc. (2009) SAS/STAT 9.2 user’s guide. SAS Institute Cary, Cary

    Google Scholar 

  • Sayed H, Backes G, Kayyal H, Yahyaoui A, Ceccarelli S, Grando S, Jahoor A, Baum M (2004) New molecular markers linked to qualitative and quantitative powdery mildew and scald resistance genes in barley for dry areas. Euphytica 135:225–228

    Article  CAS  Google Scholar 

  • Siahsar BA, Peighambari SA, Taleii AR, Naghavi MR, Nabipour A, Sarrafi A (2009) QTL analysis of forage quality traits in barley (Hordeum vulgare L.). Cereal Res Commun 37:479–488

    Article  CAS  Google Scholar 

  • Spaner D, Shugar LP, Choo TM, Falak I, Briggs KG, Legge WG, Falk DE, Ullrich SE, Tinker NA, Steffenson BJ, Mather DE (1998) Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms. Crop Sci 38:843–850

    Article  Google Scholar 

  • Vafadar Shamasbi F, Jamali SH, Sadeghzadeh B, Abdollahi Mandoulakani B (2017) Genetic mapping of quantitative trait loci for yield-affecting traits in a barley doubled haploid population derived from Clipper × Sahara 3771. Front Plant Sci 8:688. https://doi.org/10.3389/fpls.2017.00688

    Article  PubMed  PubMed Central  Google Scholar 

  • Varshney RK, Marcel TC, Ramsay L, Russell J, Roder MS, Stein N, Waugh R, Langridge P, Niks RE, Graner A (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theor Appl Genet 114:1091–1104

    Article  CAS  Google Scholar 

  • Von Korff M, Wang H, Léon J, Pillen K (2006) AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp spontaneum). Theor Appl Genet 112:1221–1231

    Article  Google Scholar 

  • Wagner C, Krämer M, Badani AG, Friedt W, Ordon F (2004) Chemical induced resistance (CIR) and detection of QTL for resistance against Rhynchosporium secalis in barley (Hordeum vulgare L.). In: Spunar J, Janikova J (eds) Proceedings of the 9th international barley genetics symposium (IBGS 2004). Kromeriz, Czech Republic, pp 742–747

  • Wang Y, Gupta S, Wallwork H, Zhang X, Zhou G, Broughton S, Loughman R, Lance R, Wu D, Shu X, Li C (2014) Combination of seedling and adult plant resistance to leaf scald for stable resistance in barley. Mol Breed 34:2081–2089

    Article  CAS  Google Scholar 

  • Yan J, Zhu J, He C, Benmoussa M, Wu P (1999) Molecular marker-assisted dissection of genotype × environment interaction for plant type traits in rice (Oryza sativa L.). Crop Sci 39:538–544

    Article  Google Scholar 

  • Zhan J, Fitt BDL, Pinnschmidt HO, Oxley SJP, Newton AC (2008) Resistance, epidemiology, and sustainable management of Rhynchosporium secalis populations on barley. Plant Pathol 57:1–14

    Google Scholar 

Download references

Acknowledgements

The authors thank the company Florimond Desprez of seed production (France) for doubled-haploid barley lines production.

Author information

Authors and Affiliations

  1. National Agronomic Institute of Tunisia, University of Carthage, Tunis, Tunisia

    W. Fériani, S. Rezgui & M. Cherif

Authors
  1. W. Fériani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Rezgui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Cherif
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Cherif.

Additional information

Communicated by A. Börner.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fériani, W., Rezgui, S. & Cherif, M. Detection of QTL and QTL × environment interaction for scald resistance in a two-row × six-row cross of barley. CEREAL RESEARCH COMMUNICATIONS 48, 187–193 (2020). https://doi.org/10.1007/s42976-020-00024-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42976-020-00024-1

Keywords

Search

Navigation