Abstract
Root lengthening is regarded as a strategy for rice to adapt to the low phosphorus level. Namely, by increasing the length and surface area of root, rice has an opportunity to enhance its phosphorus adsorption efficiency under low phosphorus conditions. The relationship between main root lengthening of rice seedlings under low phosphorus conditions with the endogenous hormone contents and expression of expansin genes were examined by using methods of the hormone content determination and transcriptome sequencing. The experimental results suggested that after low phosphorus treatment for 15 days, the main root length of Tongjing981 (TJ981) and Zhendao99 (ZD99) was considerably longer than that of the control. However, the main root length of Zhenghan6 (ZH6) was shorter compared to the control. It reveals that the main root length of different rice varieties had different response modes to low phosphorus treatment. After low phosphorus treatment, the IAA content of TJ981 and ZD99 had dramatically increased IAA contents compared to the control, while the IAA content in the root system of ZH6 evidently decreased compared to the control. This finding indicates that the response mode of the IAA content in the roots of three rice varieties for the test to low phosphorus treatment was consistent with that of the main root length. Meanwhile, low-concentrated (5 mg L−1) exogenous IAA markedly elongated the main root of TJ981. Consequently, a higher IAA content in rice roots than that of the control may result in lengthening the rice main root induced by low phosphorus treatment. Low phosphorus treatment induced the expression of multiple expansin genes in TJ981 and ZD99 roots; meanwhile, exogenous IAA treatment at various doses also promoted the expression of expansin genes. Hence, the expression of expansin genes in rice roots induced by low phosphorus treatment was related to the increased IAA content caused by low phosphorus stress. Moreover, low phosphorus treatment promoted the length of cells in the elongation zone of TJ981 compared with the control.
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The data sets generated and analyzed during the current study are available from the corresponding author on reasonable request.
References
Cao SQ, Chen ZY, Liu GQ et al (2009) The Arabidopsis ethylene-insensitive 2 gene is required for lead resistance. Plant Physiol Biochem 47(4):308–312. https://doi.org/10.1016/j.plaphy.2008.12.013
Chen AG, Chen JH (2003) Advances in the study of expansin. Chin Bull Bot 20(6):752–758
Ding Y, Wang ZG, Ren ML et al (2018) Iron and callose homeostatic regulation in rice roots under low phosphorus. BMC Plant Biol 18:326–340. https://doi.org/10.1186/s12870-018-1486-z
Dai X, Wang Y, Yang A et al (2012) OsMYB2P-1, an R2R3 MYB transcription factor, is involved in the regulation of phosphate-starvation responses and root architecture in rice. Plant Physiol 159:169–183. https://doi.org/10.1104/pp.112.194217
Dai X, Wang Y, Zhang WH (2016) OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice. J Exp Bot 67(3):947–960. https://doi.org/10.1093/jxb/erv515
Gutiérrez-Alanís D, Yong-Villalobos L, Jiménez-Sandoval P et al (2017) Phosphate starvation-dependent iron mobilization induces CLE14 expression to trigger root meristem differentiation through CLV2/PEPR2 signaling. Dev Cell 41(5):555–570. https://doi.org/10.1016/j.devcel.2017.05.009
Gamuyao R, Chin JH, Pariasca-Tanaka J et al (2012) The protein kinase pstol1 from traditional rice confers tolerance of phosphorus deficiency. Nature 488(7412):535–539. https://doi.org/10.1038/nature11346
Guo W, Zhao J, Li X et al (2011) A soybean β-expansin gene GmEXPB2 intrinsically involved in root system architecture responses to abiotic stresses. Plant J 66:541–552. https://doi.org/10.1111/j.1365-313X.2011.04511.x
Gao B, Cao CL, Li T (2012) Effect of ethylene on morphology and physiological characteristic of soybean seedlings under low-phosphorus stress. Soybean Science 31(1):58–63
Huang R, Sun HW, Liu SJ et al (2012) Rice root growth and auxin concentration in response to phosphate deficiency. Chin J Rice Sci 26(5):563–568. https://doi.org/10.3969/j.issn.1001-7216.2012.05.008
Huang WN, Kang YF (2013) Research advances in ethylene regulation to growth and development of seedling root. Chin Agric Sci Bull 29(12):6–12
Hutchison KW, Singer PB, Mclnnis S et al (1999) Expansins are conserved in conifers and expressed in hypocotyls in response to exogenous auxin. Plant Physiol 120:827–831. https://doi.org/10.1104/pp.120.3.827
Hua YJ, Wang SN, Liu ZX et al (2016) Transcriptomic analysis of Pseudostellariae Radix from different fifields using RNA-seq. Gene 588:7–18. https://doi.org/10.1016/j.gene.2016.04.043
Li F, Pan XH, Liu SY et al (2004) Effect of phosphorus deficiency stress on root morphology and nutrient absorption of rice cultivars. Acta Agron Sinica 30(5):438–442
Li JZ, Xie Y, Dai AY et al (2009) Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice. J Genet Genom 36(3):173–183. https://doi.org/10.1016/S1673-8527(08)60104-6
Li HB, Xia M, Wu P (2001) Effect of phosphorus deficiency stress on rice lateral root growth and nutrient absorption. Acta Bot Sin 43(11):1154–1160
Li F, Li FL, Yao SK et al (2017) Research progress of the effect of expansions on the root formation. Plant Physiol J 53(2):174–184
Müller J, Toev T, Heisters M et al (2015) Iron-dependent callose deposition adjusts root meristem maintenance to phosphate availability. Dev Cell 33:216–230. https://doi.org/10.1016/j.devcel.2015.02.007
Petricka JJ, Winter CM, Benfey PN (2011) Control of Arabidopsis root development. Annu Rev Plant Biol 63(1):563–590. https://doi.org/10.1146/annurev-arplant-042811-105501
Shimizu A, Yanagihara S, Kawasaki S et al (2004) Phosphorus deficiency-induced root elongation and its QTL in rice (Oryza sativa L.). Theor Appl Genet 109(7):1361–1368. https://doi.org/10.1007/s00122-004-1751-4
Shimizu A, Kato K, Komatsu A et al (2008) Genetic analysis of root elongation induced by phosphorus deficiency in rice (Oryza sativa L.): fine QTL mapping and multivariate analysis of related traits. Theor Appl Genet 117(6):987–996. https://doi.org/10.1007/s00122-008-0838-8
Shen C, Wang S, Zhang S et al (2013) OsARF16, a transcription factor, is required for auxin and phosphate starvation response in rice (Oryza sativa L.). Plant Cell Environ 36:607–620. https://doi.org/10.1111/pce.12001
Sun XD, Yang HQ, Wei SC et al (2008) Cloning and expression of a full-length cDNA of expansin gene from new root of Malus hupehensis Rehd. Sci Agric Sin 41(5):1548–1553. https://doi.org/10.3864/j.issn.0578-1752.2008.05.040
Wasaki J, Yamamura T, Shinano T et al (2003) Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency. Plant Soil 248(1–2):129–136. https://doi.org/10.1023/A:1022332320384
Wei W, Yang C, Luo J et al (2010) Synergism between cucumber α-expansin, fungal endoglucanase and pectin lyase. J Plant Physiol 167(14):1204–1210. https://doi.org/10.1016/j.jplph.2010.03.017
Wen-Wen YH, Huang JL, Zou HY, et al (2013) The effects of expansins on growth and development of plant root system. Chinese Bull of Life Sci 25(9):922–931. https://doi.org/10.13376/j.cbls/2013.09.015
Yang WT, Baek D, Yun D et al (2014) Overexpression of OsMYB4P, an R2R3-type MYB transcriptional activator, increases phosphate acquisition in rice. Plant Physiol Bioch 80:259–267. https://doi.org/10.1016/j.plaphy.2014.02.024
Yi K, Wu Z, Zhou J et al (2005) OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol 138:2087–2096. https://doi.org/10.1104/pp.105.063115
Zhao YJ, Li LG (2011) Plant cell wall loosening factors. Plant Physiol J 47(10):925–935. https://doi.org/10.12688/f1000research.7180.1
Zhou J, Jiao FC, Wu ZC et al (2008) OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol 146(4):1673–1686. https://doi.org/10.1104/pp.107.111443
Zhou DX, Yin K, Xu ZH et al (2003) Effect of polar auxin transport on rice root development. Acta Botanica Sinica 45(12):1421–1427
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A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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This research was supported by the Special Fund for Agro-Scientific Research in the Public Interest (No. 201103007), the National Natural Science Foundation Item (No. 31671618), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.
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All authors contributed to the study conception and design. CG conceived the study, edited the manuscript, and supervised the work. YW participated in conceiving the project, provided financial support for the study, and supervised the work. YD carried out most experimentation, contributed to the design of the study, and drafted the manuscript. ZW carried out most transcriptome and protein spectrometry data analysis. Material preparation was performed by SM, JL, YX, and YW. All authors read and approved the final manuscript.
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Ding, Y., Wang, Z., Mo, S. et al. Mechanism of Low Phosphorus Inducing the Main Root Lengthening of Rice. J Plant Growth Regul 40, 1032–1043 (2021). https://doi.org/10.1007/s00344-020-10161-w
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DOI: https://doi.org/10.1007/s00344-020-10161-w