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Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda

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Abstract

The ridgetail white prawn Exopalaemon carinicauda has the potential to be used as a model organism in crustacean research because it has a transparent body, available draft genome, and short life cycle. However, their ovarian development pattern remains unclear under laboratory culture conditions. This study investigated the changes of ovarian external feature, ovarian histology, gonadosomatic index (GSI), and hepatosomatic index (HSI), as well as the expression and localization of vitellogenin in the ovary and the hepatopancreas during the first ovarian development cycle of E. carinicauda under laboratory-reared condition. The results demonstrated that (1) the first ovarian development cycle of E. carinicauda could be divided into 5 different stages in which the ovary changes its color from white to yellow during the vitellogenesis process in parallel with increasing GSI. (2) After pubertal molt, most females reached ovarian stage II while the females reached stage V after premating molt. (3) During the ovarian development, GSI increased smoothly and HSI relatively stable during the period of stages I to IV, while GSI increased but HSI decreased significantly from stages IV to V. (4) In situ hybridization (ISH) revealed that EcVg was slightly expressed in the oocyte cytoplasm of previtellogenic oocytes. The positive signal was mainly detected in hepatopancreatic fibrillar cells, and a strong signal was found in the hepatopancreas at stage IV. Moreover, the expression level of EcVg-mRNA in the hepatopancreas is stage-specific, and the hepatopancreas contributes majority of vitellin precursor protein to support the ovarian development of E. carinicauda.

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Acknowledgments

We gratefully thank Mrs. Guiping Pan and Wenjie Hou from the Shanghai Fisheries Institute for their assistance in sampling. Sincere thanks are also due to Miss Meimei Liu from the Shanghai Ocean University for her help and valuable suggestions.

Funding

This work was supported by a Key R&D Program (No. 2018YFD0900100) from the Ministry of Science and Technology of China and the Capacity Promotion Project of Shanghai Engineering and Technology Center from the Shanghai Municipal Science and Technology Commission (No.19DZ2284300). Infrastructure costs were supported by the construction and improvement project (No. A1-2801-18-1003) for high-level university in Shanghai from the Shanghai Education Commission and the Shanghai talent development fund for young scientists (No. 2018100) from the Shanghai Municipal Human Resources and Social Security Bureau.

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  1. Lin Wang and Qing Guo contributed equally to this work.

Authors and Affiliations

  1. Shanghai Collaborative Innovation Centre for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China

    Lin Wang, Qing Guo & Xugan Wu

  2. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    Lin Wang, Qing Guo & Xugan Wu

  3. Department of Life Sciences, Ben-Gurion University of the Negev, 84105, Beer Sheva, Israel

    Tom Levy

  4. Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Ting Chen

  5. National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China

    Xugan Wu

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  1. Lin Wang
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Correspondence to Ting Chen or Xugan Wu.

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All shrimps were treated in accordance with the guidelines for the care and use of experimental animals established by the Administration of Affairs Concerning Experimental Animals of the State Council of the People’s Republic of China, and approved by the Ethical Committee on Experimental Animal Management of the Shanghai Ocean University.

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Wang, L., Guo, Q., Levy, T. et al. Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda. Cell Tissue Res 382, 367–379 (2020). https://doi.org/10.1007/s00441-020-03223-8

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  • DOI: https://doi.org/10.1007/s00441-020-03223-8

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