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Evaluation of okra (Abelmoschus esculentus L. Moench) cultivars for resistance to okra mosaic virus and okra yellow vein mosaic virus

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Abstract

Okra (Abelmoschus esculentus L. Moench) serves as a vegetable of great food, medicinal and industrial value. The production of the crop is, however, constrained by lack of improved high yielding, virus and pest resistant varieties. The current study sought to identify resistance in ten okra cultivars to okra mosaic disease and okra yellow vein mosaic disease within the coastal savannah agro-ecological zone of Ghana. Enzyme-linked immunosorbent assay (ELISA) detected Okra mosaic virus (OkMV) and Okra yellow vein mosaic virus (OYVMV) in symptomatic leaves of the ten cultivars. Okra mosaic disease was the most frequently detected in cultivar Adom while okra yellow vein mosaic disease was most abundant in cultivar Labadi dwarf. Mixed infection of OkMV and OYVMV was found in eight cultivars while cultivars F1-Sahari and F1-Kirene were singly infected by OkMV. The highest index of symptom severity for all plants (ISSap) was found in cultivar Kwabenya (2.80) while Togo and Adom had the least (0.30). The index of symptom severity for diseased plants only (ISSdp) varied significantly and was highest in cultivar Kwabenya (3.85). Based on disease incidence and severity, cultivars Adom, Togo, Asutem, Labadi dwarf, Kirikou-F1 and Kwabenya were rated as tolerant while F1-Sahari and Lucky-19F1 were rated as moderately susceptible. F1-Kirene and Clemson Spineless were rated as highly susceptible with disease incidence of 88.6% and 88.5% respectively.

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Acknowledgments

We wish to profoundly commend the invaluable contributions of Mr. Yusif Mohammed (School of Nuclear and Allied Sciences) as well as staff of the Biotechnology Centre that have enabled the success of this work. We also acknowledge the Biotechnology and Nuclear Agriculture Research Institute (BNARI) of the Ghana Atomic Energy Commission (GAEC) for permitting the use of the Molecular Biology Laboratory and other facilities to carry out this study.

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Authors and Affiliations

  1. Biotechnology Centre, Biotechnology and Nuclear Agriculture Research Institute (BNARI), P. O. Box AE 50, Accra, Ghana

    A. S. Appiah & S. Amiteye

  2. Department of Nuclear Agriculture and Radiation Processing, School of Nuclear and Allied Sciences, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana

    F. Boateng & H. M. Amoatey

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  1. A. S. Appiah
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  2. S. Amiteye
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  3. F. Boateng
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  4. H. M. Amoatey
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Correspondence to A. S. Appiah.

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Appiah, A.S., Amiteye, S., Boateng, F. et al. Evaluation of okra (Abelmoschus esculentus L. Moench) cultivars for resistance to okra mosaic virus and okra yellow vein mosaic virus. Australasian Plant Pathol. 49, 541–550 (2020). https://doi.org/10.1007/s13313-020-00727-3

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  • DOI: https://doi.org/10.1007/s13313-020-00727-3

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