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PROTEIN UBIQUITINATION

Unraveling proteasome engagement

Nature Chemical Biology volume 16, pages 813–815 (2020)Cite this article

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A new study reveals that, in addition to its longstanding role in recruiting proteins to the proteasome, ubiquitination can also induce a structural destabilization that allows the target protein to be efficiently unraveled for degradation.

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Fig. 1: Strategy for native, site-specific ubiquitination.

References

  1. Yu, H. & Matouschek, A. Annu. Rev. Biophys. 46, 149–173 (2017).

    Article  CAS  Google Scholar 

  2. Prakash, S., Tian, L., Ratliff, K. S., Lehotzky, R. E. & Matouschek, A. Nat. Struct. Mol. Biol. 11, 830–837 (2004).

    Article  CAS  Google Scholar 

  3. Carroll, E. C., Greene, E. R., Martin, A. & Marqusee, S. Nat. Chem. Biol. https://doi.org/10.1038/s41589-020-0556-3 (2020).

  4. Hagai, T., Azia, A., Tóth-Petróczy, Á. & Levy, Y. J. Mol. Biol. 412, 319–324 (2011).

    Article  CAS  Google Scholar 

  5. Varshavsky, A., Bachmair, A., Finley, D., Gonda, D. & Wünning, I. in Ubiquitin (ed. Rechsteiner, M.) 287–324 (Springer US, 1988).

  6. Morimoto, D., Walinda, E., Fukada, H., Sugase, K. & Shirakawa, M. Sci. Rep. 6, 39453 (2016).

    Article  CAS  Google Scholar 

  7. Hagai, T. & Levy, Y. Proc. Natl. Acad. Sci. USA 107, 2001–2006 (2010).

    Article  CAS  Google Scholar 

  8. Saeki, Y., Isono, E. & Toh-E, A. Methods Enzymol. 399, 215–227 (2005).

    Article  CAS  Google Scholar 

  9. Barandun, J. et al. BMC Struct. Biol. 17, 1 (2017).

    Article  Google Scholar 

  10. Goodsell, D. S., Autin, L. & Olson, A. J. Structure 27, 1716–1720.e1 (2019).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR, USA

    Cameron G. Roberts & Jonathan N. Pruneda

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  1. Cameron G. Roberts
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  2. Jonathan N. Pruneda
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Correspondence to Jonathan N. Pruneda.

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The authors declare no competing interests.

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Roberts, C.G., Pruneda, J.N. Unraveling proteasome engagement. Nat Chem Biol 16, 813–815 (2020). https://doi.org/10.1038/s41589-020-0573-2

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