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Disturbance of oxidant/antioxidant status and impairment on fillet fatty acid profiles in Brycon amazonicus subjected to acute heat stress

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Abstract

Amazon fish are vulnerable to climate change. Current temperature increases in Amazonian rivers are likely to continue in the coming years. Elevated temperatures impair homeostasis and subject fish to oxidative stress; nevertheless, the effects of elevated temperature on plasma and muscle oxidative stress as well as fillet fatty acid composition in matrinxã (Brycon amazonicus) remain unknown. Therefore, the aims of the present study were to determine whether antioxidant system is able to withstand acute thermal stress to avoid plasma and muscle oxidative damage and to determine the manner in which matrinxã adjust their muscle fatty acid metabolism in a response to acute heat stress. We exposed juvenile matrinxã to four temperatures (28 °C as control and 30, 32, and 34 °C) for 72 h and observed the effects on plasma and muscle oxidant/antioxidant status and on fatty acid composition. Plasma reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were significantly higher in matrinxã exposed to 34 °C compared with the control group, while they were significantly higher in the muscle of matrinxã exposed to 32 °C and 34 °C compared with the control group. Superoxide dismutase (SOD) activity and total antioxidant capacity (TAC) levels were significantly lower in the plasma and muscle of matrinxã exposed to 34 °C compared with the control group. Plasma glutathione peroxidase (GPx) activity was significantly lower in matrinxã exposed to 32 and 34 °C compared with the control group, while it was significantly lower in the muscle of matrinxã exposed to 34 °C. The total content of saturated fatty acid (SFA) was significantly higher in the fillet of matrinxã exposed to 34 °C compared with the control group, while the total content of polyunsaturated fatty acid (PUFA) was significantly lower. Based on this evidence, we conclude that acute heat stress at 32 °C and 34 °C causes plasma and muscular oxidative stress, and the enzymatic and non-enzymatic antioxidant system is unable to prevent oxidative damage. Moreover, increased levels of total SFA and decreased levels of PUFA occur in an attempt to withstand heat stress and maintain membrane fluidity; nevertheless, these responses can represent negative impacts for consumers.

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Acknowledgments

The authors are grateful to the Conselho Nacional de Desenvolvimento Tecnológico (CNPq), Comissão de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, finance code 001), and INCT-ADAPTA ((CNPq, 465540/2014-7)/FAPEAM 062.1187/2017/CAPES, finance code 001).

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

    Matheus D. Baldissera, Carine F. Souza & Bernardo Baldisserotto

  2. LEEM—Laboratory of Ecophysiology and Molecular Evolution, Brazilian National Institute for Research of the Amazon, Manaus, Amazonas, Brazil

    Danilo C. Barroso & Adalberto Luis Val

  3. Department of Food Science and Technology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil

    Renata B. Falk & Roger Wagner

Authors
  1. Matheus D. Baldissera
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  2. Carine F. Souza
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  3. Danilo C. Barroso
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Correspondence to Matheus D. Baldissera.

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The Ethics Committee of INPA authorized all experimental procedures under the number 004/2018, SEI 01280.000209/2018-74.

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Baldissera, M.D., Souza, C.F., Barroso, D.C. et al. Disturbance of oxidant/antioxidant status and impairment on fillet fatty acid profiles in Brycon amazonicus subjected to acute heat stress. Fish Physiol Biochem 46, 1857–1866 (2020). https://doi.org/10.1007/s10695-020-00835-3

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