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Preparation and cellular-interaction investigation of 177Lu/FITC labeled NGR peptides

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Abstract

Aminopeptidase N (CD13) as the malignant cell surface marker is overexpressed on many tumor cells including lung cancer and the neovascular cell membranes. A tripeptide structure, Asn-Gly-Arg (NGR), can specifically bind to CD13. In this study, we successfully labeled various NGR peptides derivatives with FITC or 177Lu at high efficiency. The 177Lu labeling of the NGR peptides was stable in vitro. The cellular uptake of the 177Lu-labeled cyclic NGR peptides was different by varying the structure of peptides.

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Acknowledgements

This work was funded from the National Key R&D Program of China (2017YFC1200403), the National Natural Science Foundation of China (Grant Nos. 21976167 and 21906155), and Nuclear Energy Development Project of State Administration of Science, Technology and Industry for National Defense (20181524-1).

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Authors and Affiliations

  1. Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, People’s Republic of China

    Zhikai Ding, Huaxia Fu, Xiangyu Li, Wei Liao, Yuchuan Yang, Hongyuan Wei & Yue Chen

  2. Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, 621900, People’s Republic of China

    Zhikai Ding, Huaxia Fu, Xiangyu Li, Jing Wang, Liangang Zhuo, Wei Liao, Yuchuan Yang, Hongyuan Wei, Xia Yang & Yue Chen

  3. Collaborative Innovation Center of Radiation Medicine of Jiangsu, Higher Education Institutions, Suzhou, 215123, People’s Republic of China

    Jing Wang, Liangang Zhuo & Xia Yang

  4. Key Laboratory of Nuclear Medicine and Molecular Imaging of Sichuan Province, Mianyang, 621999, People’s Republic of China

    Jing Wang, Liangang Zhuo & Xia Yang

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  1. Zhikai Ding
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  2. Huaxia Fu
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  8. Hongyuan Wei
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  9. Xia Yang
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  10. Yue Chen
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Correspondence to Hongyuan Wei, Xia Yang or Yue Chen.

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Ding, Z., Fu, H., Li, X. et al. Preparation and cellular-interaction investigation of 177Lu/FITC labeled NGR peptides. J Radioanal Nucl Chem 325, 67–74 (2020). https://doi.org/10.1007/s10967-020-07223-4

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  • DOI: https://doi.org/10.1007/s10967-020-07223-4

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