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A new fluorescence sensor developed with polydopamine nanospheres for the detection of anti-PLA2R antibody biomarkers of idiopathic membranous nephropathy

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Abstract

Anti-PLA2R antibody is only expressed in podocytes from patients with idiopathic membranous nephropathy (IMN). The detection of anti-PLA2R antibody in serum is therefore able to obtain essential information for rapid diagnosis and evaluation of the disease activity of IMN. In the present study, a polydopamine nanosphere-based fluorescent sensor was constructed for direct detection of anti-PLA2R antibodies in human serum. In this sensing system, the double-stranded DNA was phosphorylated under the action of anti-PLA2R antibody and the single-stranded DNA was cut by exonuclease. The single-stranded DNA was then adsorbed on polydopamine microspheres. The fluorescent groups labeled on the DNA were quenched, and the concentration of anti-PLA2R antibody was detected quantitatively by measuring the fluorescence signal changes before and after the reaction. The experimental results show that the method has a good linear detection range between 0.05 and 10 μg/mL for anti-PLA2R antibody and the detection limit is 0.02 μg/mL.

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Funding

This study was funded by the financial support of Nature Sciences Funding of Fujian Province (2018J01293).

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Author notes

  1. Linlin Li and Heng Lu contributed equally to this work.

Authors and Affiliations

  1. Department of Nephrology, Union Hospital, Fujian Medical University, Fuzhou, China

    Linlin Li, Hong Ye, Qi Zou, Qiaoling Chen & Lixin Wei

  2. Department of Cardiac Surgery, Union Hospital, Fujian Medical University, Fuzhou, China

    Heng Lu

  3. Department of Laboratory Medicine, Union Hospital, Fujian Medical University, Fuzhou, China

    Jingxi Zhang

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  1. Linlin Li
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  2. Heng Lu
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  3. Hong Ye
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  4. Qi Zou
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  5. Qiaoling Chen
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  6. Lixin Wei
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Correspondence to Lixin Wei or Jingxi Zhang.

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Li, L., Lu, H., Ye, H. et al. A new fluorescence sensor developed with polydopamine nanospheres for the detection of anti-PLA2R antibody biomarkers of idiopathic membranous nephropathy. Anal Bioanal Chem 412, 4549–4554 (2020). https://doi.org/10.1007/s00216-020-02703-8

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  • DOI: https://doi.org/10.1007/s00216-020-02703-8

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