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Molybdenum Application Regulates Oxidative Stress Tolerance in Winter Wheat Under Different Nitrogen Sources

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Abstract

Molybdenum (Mo), an essential microelement, may enhance the oxidative stress tolerance in plants. However, the efficacy of Mo might be variable with different forms of nitrogen (N) fertilizer. The present study was conducted to investigate the role of Mo application in regulating oxidative stress tolerance in winter wheat under different N sources. A hydroponic study was carried out comprising of two winter wheat cultivars ‘97003’ and ‘97014’ as Mo efficient and Mo inefficient, respectively, under two Mo levels (0 and 1 μM) and three different N sources (NO3̶, NH4NO3, or NH4+). Winter wheat plants supplied with different N sources accumulated superoxide anions (O2−), and malonaldehyde (MDA) contents in the order of NH4+ > NO3̶ > NH4NO3, suggesting that sole application of either N sources, especially sole NH4+ source, may induce oxidative stress in winter wheat. However, Mo application decreased the MDA contents by 20.02%, 15.11%, and 25.89% in Mo-efficient cultivar and 30.75%, 23.79%, and 37.76% in Mo-inefficient cultivar under NO3̶, NH4NO3, and NH4+ sources, respectively, while increased antioxidant enzyme activities and carotenoids and abscisic acid (ABA) contents up-regulated the expressions of TaAO and TaAba3 genes. Mo application regulated oxidative stress tolerance in winter wheat under different N sources through enhancing ABA production and ROS-scavenging enzymes. Mo-efficient ‘97003’ winter wheat cultivar possesses a wider range of adaptability to withstand Mo-deficient conditions than Mo-inefficient ‘97014’ cultivar.

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Funding

This work was supported by the National Natural Science Foundation of China (Program No. 41771329) and the 948 Project from the Ministry of Agriculture of China (2016-X41).

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Authors and Affiliations

  1. Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Muhammad Imran, Xuecheng Sun, Muhammad Shoaib Rana & Chengxiao Hu

  2. Department of Crop Science and Technology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, People’s Republic of China

    Muhammad Imran

  3. Department of Agronomy, University of Agriculture Faisalabad, Sargodha, Punjab, 38040, Pakistan

    Saddam Hussain & Fahd Rasul

  4. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Usman Ali

  5. Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, Sargodha, Punjab, Pakistan

    Shadab Shaukat

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  1. Muhammad Imran
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Imran, M., Sun, X., Hussain, S. et al. Molybdenum Application Regulates Oxidative Stress Tolerance in Winter Wheat Under Different Nitrogen Sources. J Soil Sci Plant Nutr 20, 1827–1837 (2020). https://doi.org/10.1007/s42729-020-00254-6

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