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Fertility and gamete ploidy of allopentaploid offspring of tetraploid dojo loach Misgurnus anguillicaudatus females and large-scale loach Paramisgurnus dabryanus males

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Abstract

Artificial interspecific hybrids (3n) of tetraploid dojo loach Misgurnus anguillicaudatus (TTTT) and diploid large-scale loach Paramisgurnus dabryanus (PP) were viable. Allopentaploid loaches (TTTTP) were produced by cold-shocking the fertilized eggs of TTTT females fertilized with sperm of PP males. The TTTTP were viable and well developed. As there are currently no reports on the fertility and gamete ploidy of TTTTP, artificial propagation and ploidy analyses were conducted to determine them. The ovaries of TTTTP were well developed with primary oocytes and secondary oocytes; the testes were sterile with low-density sperm-like cells. By backcrossing with mature TTTT, TTTTP were found to be female fertile and male sterile. The fertility of female TTTTP was low; they produced few viable diploid eggs and a few inviable triploid and aneuploid eggs. Ploidy analysis of cold-shock-induced progenies of TTTTP × TTTT indicated that TTTTP could generate viable diploid eggs, along with diploid second polar bodies. The results of this study are helpful for further understanding of loach polyploidization and should encourage more investigations on hybridization and gamete formation in loaches.

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Acknowledgements

This study was financially supported by the National Key R&D Program of China (project number: 2018YFD0900200) and Fundamental Research Funds for the Central Universities of China (project number: 2662020SCPY002). Special thanks to Dr. Tea Tomljanovic at the University of Zagreb and Dr. Wang at the University of California Davis for revising the English of this manuscript.

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Authors and Affiliations

  1. College of Fisheries, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan, 437000, Hubei, People’s Republic of China

    Songqian Huang, Xiaojuan Cao & Weimin Wang

  2. Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan

    Songqian Huang

  3. Shanghai University of Finance and Economics, Shanghai, 200433, People’s Republic of China

    Li Zhang

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  1. Songqian Huang
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  2. Li Zhang
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  3. Xiaojuan Cao
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  4. Weimin Wang
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Correspondence to Xiaojuan Cao.

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Huang, S., Zhang, L., Cao, X. et al. Fertility and gamete ploidy of allopentaploid offspring of tetraploid dojo loach Misgurnus anguillicaudatus females and large-scale loach Paramisgurnus dabryanus males. Fish Sci 86, 741–748 (2020). https://doi.org/10.1007/s12562-020-01432-2

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  • DOI: https://doi.org/10.1007/s12562-020-01432-2

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