Abstract
Objectives
To evaluate a strain of Fusarium verticillioides ITV03 isolated from wood residues in the Veracruz region of Mexico. Endoglucanase and β-glucosidase production by submerged fermentation was optimized using a Box–Behnken design, where the independent variables were urea, ammonium sulfate and yeast extract.
Results
After optimization, an endoglucanase activity of 0.27 U/mL was achieved; subsequently, three carbon sources were evaluated (carboxymethyl cellulose, sweet sorghum bagasse cellulose and delignified sweet sorghum bagasse (DSSB). The results showed that DSSB yielded the greatest endoglucanase (0.28 U/mL) and β-glucosidase (0.12 U/mL) activities. Both enzymatic activities were characterized for the effect of pH, temperature and thermostability. The optimal parameters of β-glucosidase and endoglucanase activity were pH 5 and 4 respectively, the optimum temperature 60 °C. These enzymes were stable at 50 °C for 150.68 h and 8.54 h, with an activation energy (Ea(day)) of 265.55 kJ/mol and 44.40 kJ/mol respectively, for β-glucosidase and endoglucanase.
Conclusion
The present work shows that a native strain like F. verticillioides ITV03 using DSSB supplemented with nitrogen has a great potential as a producer of cellulase for lignocellulosic residue hydrolysis.
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Acknowledgements
The authors acknowledge the economic support from the Sectoral Fund for Research in Agricultural, Livestock, Aquaculture, Agrobiotechnology and Plant Genetic Resources and the National Council of Science and Technology, Mexico (SAGARPA-CONACyT, Project 291143 and PN-4650) and the TecNM, project 6853.18P; also the critical reading of Patricia Margaret Hayward-Jones, M Sc and Dulce María Barradas-Dermitz, M Sc.
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Infanzón-Rodríguez, M.I., Ragazzo-Sánchez, J.A., del Moral, S. et al. Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusarium verticillioides ITV03 using sweet sorghum bagasse. Biotechnol Lett 42, 2271–2283 (2020). https://doi.org/10.1007/s10529-020-02940-y
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DOI: https://doi.org/10.1007/s10529-020-02940-y