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Antigen Recognition Patterns of Intestinal Capillariasis Using Immunoblot-Based Serodiagnosis

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Abstract

Background

Diagnosis of intestinal capillariasis depending on microscopic detection of parasitic stages is of low sensitivity, especially in cases with low worm burden. There is a necessity to develop sensitive and specific diagnostic tools of capillariasis for early treatment to avoid complications. Western blot (WB) technique showed promising results for antigen recognition patterns in several parasitic infections.

Aim of the Study

This study is directed to identify and evaluate relevant proteins of intestinal capillariasis crude worm antigens using WB immunodiagnosis.

Materials and Methods

Capillaria crude worm antigens were extracted and analyzed using SDS-PAGE. Sixty serum samples belonging to 3 groups (20 individuals each) were included; Group I (shedding Capillaria in feces), Group II (infected with other parasites) and Group III (negative parasitological results). Reactivity of the resulting bands of Capillaria with serum samples was analyzed using WB technique.

Results

Thirty-two immunoreactive bands were detected in WB analysis representing recognition of proteins with molecular weights (MW) varying from 19 to 110 kDa. Immunodominant proteins of 23.5, 31, 36.5, 40.5 and 44 kDa were recognized, respectively, in 35%, 30%, 85%, 95% and 75% of sera from patients with confirmed capillariasis and in 30%, 25%, 35%, 25%, and 20% of sera from those infected with other parasitic infections. One serum sample from group III gave reaction with 31 kDa band.

Conclusion

Immunodiagnosis of intestinal capillariasis using WB proved that 23.5, 31, 36.5, 40.5 and 44 kDa bands could be considered useful tools for diagnosis of capillariasis.

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Authors and Affiliations

  1. Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, El-Manial, Cairo, Egypt

    Marwa A. Hassan, Maha M. A. Basyoni & Abeer Said Abdel- Ghany Al-Antably

  2. Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Marwa F. Amer

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  1. Marwa A. Hassan
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  2. Maha M. A. Basyoni
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  3. Marwa F. Amer
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  4. Abeer Said Abdel- Ghany Al-Antably
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All manuscript authors contributed to every activity of it; idea of paper, study design, collection of materials, methodology, writing the paper and revising it.

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Correspondence to Abeer Said Abdel- Ghany Al-Antably.

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The authors declare that they have no competing interests.

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All procedures were explained to patients or their parents/guardians on behalf of their children and informed consents were obtained. The research was approved from the Scientific Research Ethical Committee, Faculty of Medicine Cairo University.

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Hassan, M.A., Basyoni, M.M.A., Amer, M.F. et al. Antigen Recognition Patterns of Intestinal Capillariasis Using Immunoblot-Based Serodiagnosis. Acta Parasit. 65, 899–905 (2020). https://doi.org/10.2478/s11686-020-00233-y

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