Abstract
A chitinolytic bacterium Chitinophaga sp. S167 producing extracellular chitinases was isolated from a soil sample in India. The extracellular chitinases produced by S167 were concentrated by ammonium sulphate precipitation (AS70) and seven bands corresponding to chitinases were observed by zymography. Optimum temperature and pH of AS70 were between 40 and 45 °C and pH 6.0 respectively with high stability at 20–40 °C and pH 5–7. AS70 inhibited the growth of Fusarium oxysporum, Alternaria alternata and Cladosporium sp. in vitro. The culture conditions for the high level production of extracellular chitinases were optimized resulting in 48-folds higher chitinase production. As the combination of chitinases could be more potent in biocontrol of plant diseases, it was checked if AS70 could control postharvest fungal infection caused by Fusarium oxysporum on tomatoes. AS70 treated tomatoes showed significant lower incidence of infection (11%) by F. oxysporum as compared with 100% in the control at 5 days post inoculation. Further, AS70 caused significant mortality in second stage juveniles of root knot nematode, Meloidogyne incognita, a major agriculture pest responsible for economic losses in agriculture. This study highlights the antifungal and nematicidal activity of chitinases produced by Chitinophaga sp. S167. To the best of our knowledge, this is the first report of the biocontrol potential of the chitinases produced by Chitinophaga sp.
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Conceived and designed the experiments: RK, PO. Performed the experiments: Chitinase experiments-SS, Nematicidal Activity-AK, Strain isolation-SK. Analyzed the data: RK, RK, PO. Wrote the manuscript: SS, AK (initial draft), RK (final draft).
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Sharma, S., Kumar, S., Khajuria, A. et al. Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167. World J Microbiol Biotechnol 36, 90 (2020). https://doi.org/10.1007/s11274-020-02864-9
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DOI: https://doi.org/10.1007/s11274-020-02864-9