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Parvalbumin immunohistochemical expression in the spectrum of perivascular epithelioid cell (PEC) lesions of the kidney

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Abstract

Parvalbumin is a cytosolic calcium-binding protein expressed in the distal convoluted tubule of the renal nephron. Among epithelial renal tumors, the reactivity for parvalbumin is observed in chromophobe renal cell carcinomas and frequently in oncocytomas. On the other hand, there are no data available on parvalbumin expression in the mesenchymal tumors of the kidney. Therefore, the purpose of this study was to evaluate the expression of parvalbumin in the spectrum of PEC (perivascular epithelioid cells) lesions of the kidney. Sixty-six PEC lesions (37 classic angiomyolipomas, 10 microscopic angiomyolipomas, 7 epithelioid angiomyolipomas/pure epithelioid PEComas, 5 leiomyoma-like angiomyolipomas, 3 lipoma-like angiomyolipomas, 2 intraglomerular lesions, 1 angiomyolipoma with epithelial cysts (AMLEC), and 1 sclerosing angiomyolipoma) were immunohistochemically stained with parvalbumin. Overall, parvalbumin immunostain was found in fifty-six PEC lesions (85%) and absent in the remaining ten cases (15%). Classic angiomyolipomas were positive in almost all cases (97%). Intraglomerular lesions and AMLEC showed parvalbumin immunolabeling as well. None of the 7 epithelioid angiomyolipomas/pure epithelioid PEComas or the only sclerosing angiomyolipoma expressed parvalbumin. In conclusion, we demonstrated the immunolabeling of parvalbumin in almost all PEC lesions of the kidney, but not in the epithelioid angiomyolipoma/pure epithelioid PEComa. This finding could shed light on some biological characteristics observed in the PEC lesions such as the plasticity of their cellular component. Moreover, parvalbumin may be another useful tool in the differential diagnosis among epithelioid angiomyolipoma/pure epithelioid PEComa with other renal eosinophilic tumors, such as oncocytoma and chromophobe renal cell carcinoma.

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Author notes

  1. A. Caliò and S. Ammendola share first authorship and contributed equally to this article

Authors and Affiliations

  1. Department of Diagnostic and Public Health, Section of Pathology, University of Verona, Largo L. Scuro 10, 37134, Verona, Italy

    Anna Caliò, Serena Ammendola, Matteo Brunelli, Serena Pedron & Guido Martignoni

  2. Department of Pathology, Pederzoli Hospital, Peschiera del Garda, Verona, Italy

    Stefano Gobbo & Guido Martignoni

Authors
  1. Anna Caliò
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  2. Serena Ammendola
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  3. Matteo Brunelli
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  4. Serena Pedron
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  5. Stefano Gobbo
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  6. Guido Martignoni
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Contributions

A Caliò and G. Martignoni: designed and coordinated the study. A. Caliò, S. Ammendola, G. Martignoni, M. Brunelli, and S.Gobbo: collected the samples. A. Caliò, S. Ammendola, G. Martignoni, M. Brunelli, S. Gobbo and S. Pedron: performed the histopathological and immunohistochemical analyses. A. Caliò, S. Ammendola, and G. Martignoni: wrote the paper. All the authors approved the final version of the manuscript.

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Correspondence to Guido Martignoni.

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The study was conducted in accordance with the Helsinki Declaration.

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The authors declare that they have no conflict of interest.

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Caliò, A., Ammendola, S., Brunelli, M. et al. Parvalbumin immunohistochemical expression in the spectrum of perivascular epithelioid cell (PEC) lesions of the kidney. Virchows Arch 478, 785–791 (2021). https://doi.org/10.1007/s00428-020-02856-y

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  • DOI: https://doi.org/10.1007/s00428-020-02856-y

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