Abstract
Background
Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale.
Objective
To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs).
Methods
The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated.
Results
TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%).
Conclusion
TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.
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Acknowledgements
We are grateful to the departamento de posgrado de la Escuela Superior de Medicina-Instituto Politécnico Nacional and Consejo Nacional de Ciencia y Tecnología (CONACyT, CV# 744347 and transcript# A160392). This study received financial support from BEIFI and SIP (project #20180261).
Funding
CONACyT, CV# 744347 and transcript# A160392, BEIFI and SIP project #20180261.
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The authors contribute as follows: PVAB: technical work and writing, PBA: direction of the work, writing and review of the manuscript, RGMP: supervision of the technical work and writing, LMR: supervision of the technical work and writing, AGJ: writing and review of the manuscript, GGI: supervision of the technical work, writing and chief of the work.
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The current protocol was approved by the Ethics in Research Committee (CEI-02/31-05-2017) and Biosecurity Committee (CBS-06/11-09-2017) of the Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.
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Adriana-Berenice, PV., Alberto, PB., del Pilar, RG.M. et al. Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells. Mol. Cell. Toxicol. 16, 321–330 (2020). https://doi.org/10.1007/s13273-020-00084-8
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DOI: https://doi.org/10.1007/s13273-020-00084-8