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Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells

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Abstract

Background

Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale.

Objective

To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs).

Methods

The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated.

Results

TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%).

Conclusion

TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.

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Acknowledgements

We are grateful to the departamento de posgrado de la Escuela Superior de Medicina-Instituto Politécnico Nacional and Consejo Nacional de Ciencia y Tecnología (CONACyT, CV# 744347 and transcript# A160392). This study received financial support from BEIFI and SIP (project #20180261).

Funding

CONACyT, CV# 744347 and transcript# A160392, BEIFI and SIP project #20180261.

Author information

Author notes

  1. Peralta-Vega Adriana-Berenice and Parra-Barrera Alberto participated equally.

Authors and Affiliations

  1. Lab. de Medicina Regenerativa y Estudios en Cáncer, Departamento de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón S/N, Col. Casco de Santo Tomas, Delegación Miguel Hidalgo, C.P. 11340, Mexico City, CDMX, Mexico

    Peralta-Vega Adriana-Berenice, Parra-Barrera Alberto & Gisela Gutiérrez-Iglesias

  2. Instituto Nacional de Cancerología SSA, Mexico City, Mexico

    Ramos-Godínez María del Pilar

  3. Instituto Nacional de Cardiología “Ignacio Chávez” SSA, Mexico City, Mexico

    López-Marure Rebeca

  4. Hospital Infantil de México “Federico Gómez”, SSA, Mexico City, Mexico

    Arellano-Galindo José

Authors
  1. Peralta-Vega Adriana-Berenice
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  2. Parra-Barrera Alberto
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  3. Ramos-Godínez María del Pilar
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  4. López-Marure Rebeca
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  5. Arellano-Galindo José
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  6. Gisela Gutiérrez-Iglesias
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Contributions

The authors contribute as follows: PVAB: technical work and writing, PBA: direction of the work, writing and review of the manuscript, RGMP: supervision of the technical work and writing, LMR: supervision of the technical work and writing, AGJ: writing and review of the manuscript, GGI: supervision of the technical work, writing and chief of the work.

Corresponding author

Correspondence to Gisela Gutiérrez-Iglesias.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The current protocol was approved by the Ethics in Research Committee (CEI-02/31-05-2017) and Biosecurity Committee (CBS-06/11-09-2017) of the Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico.

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Adriana-Berenice, PV., Alberto, PB., del Pilar, RG.M. et al. Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells. Mol. Cell. Toxicol. 16, 321–330 (2020). https://doi.org/10.1007/s13273-020-00084-8

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