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Bioleaching of low-grade copper sulfide ore by extremely thermoacidophilic consortia at 70 °C in column reactors

极端嗜热菌群在70 °C 柱浸反应器中浸出低品位硫化铜矿

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Abstract

The effects of introducing M. sedula derivatives having different Cu2+-resistance on bioleaching capacity of a defined consortium (consisting of A. brierleyi DSM1651 and M. hakonensis HO1-1) were studied in column reactors at 70 °C. Introducing M. sedula copA mutant, a copper sensitive derivative, only had negligible effects on bioleaching. While introducing M. sedula ARS50-2, a Cu2+ resistant strain, substantially consolidated bioleaching process, with 27.77% more copper recovered after 58 d of bioleaching. Addition of M. sedula ARS50-2 likely enhanced the sulfur oxidation capacity of consortium after the 24th day under the Cu2+ stress. The majority of extreme thermoacidophiles were attached on minerals surface as indicated by quantitative PCR (qPCR) data. Successions of microbial community of extremely thermoacidophilic consortia that attached on surface of minerals were different from those in leachate. M. hakonensis HO1-1 was the dominant species attached on minerals surface in each column reactor throughout bioleaching process. The sessile M. sedula ARS50-2 remained as a major species till the 34th day. A. brierleyi DSM1651 was the most abundant planktonic species in leachate of each column reactor. These results highlight that higher Cu2+-resistance is a beneficial trait for extreme thermoacidophiles to process copper minerals.

摘要

本文研究了分别引入具有不同 Cu2+抗性的 M. sedula 菌株对极端嗜热菌群(由 A. brierleyi DSM1651 和 M. hakonensis HO1-1 组成)在 70 °C 柱浸反应器中浸出硫化铜矿的影响. 引入 Cu2+敏感的 M. sedula copA 突变菌株对硫化铜矿的浸出几乎没有影响, 铜浸出率没有提高. 然而, 引入 Cu2+ 抗性 M. sedula ARS50-2 能显著地强化浸出过程, 铜浸出率在第 58 天提高了 27.77%. 第24 天后浸出液的 pH 表明M. sedula ARS50-2 的加入能提高极端嗜热菌群的硫氧化活性. 定量 PCR 数据表明在浸出过程中, 各反应器中矿物表面与浸出液中的极端嗜热菌群群落结构不同. 极端嗜热菌在浸出过程中主要吸附在矿物表面. M. hakonensis HO 1-1 是浸出过程中各个反应器矿物表面的主要菌株. M. sedula ARS50-2 在浸出的第 34 天仍然是矿物表面的主要的菌株. A. brierleyi DSM1651 是浸出过程中各柱浸反应器浸出液中的主要菌株. 相关数据表明加入高 Cu2+抗性菌株有利于极端嗜热菌在高浓度 Cu2+胁迫 条件下对硫化铜矿的浸出.

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Authors and Affiliations

  1. School of Metallurgy and Environment, Central South University, Changsha, 410083, China

    Chen-bing Ai  (艾郴兵)

  2. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Chen-bing Ai  (艾郴兵), Yu-ting Liang  (梁昱婷), Guan-zhou Qiu  (邱冠周) & Wei-min Zeng  (曾伟民)

  3. Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha, 410083, China

    Chen-bing Ai  (艾郴兵)

  4. Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China

    Yu-ting Liang  (梁昱婷), Guan-zhou Qiu  (邱冠周) & Wei-min Zeng  (曾伟民)

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  1. Chen-bing Ai  (艾郴兵)
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  2. Yu-ting Liang  (梁昱婷)
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Correspondence to Wei-min Zeng  (曾伟民).

Additional information

Foundation item: Project(207154) supported by the Postdoctoral Research Funding of Central South University, China; Projects(31470230, 51320105006, 51604308) supported by the National Natural Science Foundation of China; Project (2017RS3003) supported by the Youth Talent Foundation of Hunan Province, China; Project(2018JJ2486) supported by the Natural Science Foundation of Hunan Province, China; Project(2018WK2012) supported by the Key Research and Development Projects in Hunan Province, China

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Ai, Cb., Liang, Yt., Qiu, Gz. et al. Bioleaching of low-grade copper sulfide ore by extremely thermoacidophilic consortia at 70 °C in column reactors. J. Cent. South Univ. 27, 1404–1415 (2020). https://doi.org/10.1007/s11771-020-4376-0

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  • DOI: https://doi.org/10.1007/s11771-020-4376-0

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