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Changes of microbial diversity during pyrite bioleaching

黄铁矿生物浸出过程中微生物的多样性变化

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Abstract

Microorganisms, one of the key factors affecting the bioleaching process, change the components of extracellular polymeric substance (EPS) and community structure to survive in leaching environments. In this work, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD) and 16S rDna high-throughput sequence analyses were used to reveal the microbial changes in planktonic and sessile phases during bioleaching. The results showed the occupation of sessile cells decreased from 66.2% to (10±3)%. After bioleaching, the planktonic and sessile cells have similar EPS, but they are different from the original cells. Pyrite dissolution mainly occurs at the early and late stages with the decreasing of particle diameter, by 50% and 40%, respectively. The 16S rDna gene based sequence analysis results in total of 1117420 Reads across the six samples, presented among 7 phyla, 9 classes, 17 orders, 23 families and 31 genera. Genera Leptospirillum and Sulfobacillus are the main bacteria at the early and middle stages, and Leptospirillum is the main genus at the end of bioleaching. Aquabacterium and Acidovorax are special genera in sessile cells and Weissella is special in planktonic ones.

摘要

微生物是影响生物浸出过程的主要因素之一, 它通过改变胞外高分子物质(EPS)的组分和群落结构来适应浸出环境. 本文利用傅里叶变换红外光谱(FTIR)、 X 射线粉末衍射(XRD)和 16S rDNA 高通量序列分析, 揭示了生物浸出过程中游离和吸附微生物群落的变化. 结果表明, 吸附微生物由 66.2% 下降到(10±3)%. 生物浸出后的游离和吸附微生物具有相似的 EPS, 但与原始状态不同. 黄铁矿溶解主要发生在早期和晚期, 随着粒度的减小, 分别减少了 50%和 40%. 基于 16S rDNA 基因的序列分析结果显示, 6 个样品共有 1117420 个 Reads, 分布在 7 个门、 9 个纲、 17 个目、 23 个科、31 个属. Leptospirillum 和 Sulfobacillus 是早期和中期的主要菌种, Leptospirillum 是后期的主要菌属. Aquabacterium 和 Acidovorax 在吸附细胞中是特殊的属, 而 Weissella 是游离细胞中独有的.

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Authors and Affiliations

  1. School of Metallurgy, Northeastern University, Shenyang, 110819, China

    Lu Yin  (殷璐), Hong-ying Yang  (杨洪英), Xiang Li  (李想), Lin-lin Tong  (佟琳琳), Zhe-nan Jin  (金哲男) & Qin Zhang  (张勤)

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  1. Lu Yin  (殷璐)
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  2. Hong-ying Yang  (杨洪英)
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  4. Lin-lin Tong  (佟琳琳)
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  6. Qin Zhang  (张勤)
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Correspondence to Hong-ying Yang  (杨洪英).

Additional information

Foundation item: Project(U1608254) supported by the Special Fund for the National Natural Science Foundation of China; Projects(ZJKY2017(B)KFJJ01, ZJKY2017(B)KFJJ02) supported by the Zijin Mining Group Co., Ltd., China

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Yin, L., Yang, Hy., Li, X. et al. Changes of microbial diversity during pyrite bioleaching. J. Cent. South Univ. 27, 1477–1483 (2020). https://doi.org/10.1007/s11771-020-4383-1

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