Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction

Marcelino Varona; Olga I. Shimelis; Jared L. Anderson

doi:10.1039/D0AY00980F

Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction†

Marcelino Varona,^a Olga I. Shimelis^b and Jared L. Anderson

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Iowa State University, 1605 Gilman Hall, Ames, Iowa 50011, USA
E-mail: andersoj@iastate.edu

^b MilliporeSigma, Bellefonte, Pennsylvania 16823, USA

Abstract

Selective DNA extraction is immensely useful for the isolation and detection of low-abundance sequences. Oligonucleotide-modified substrates are often used to capture sequences of interest for downstream analysis. In this study, we explore the chemical modification of commercial-available polyacrylate solid-phase microextraction fibers for selective DNA analysis using carbodiimide crosslinker chemistry. Reproducible modification conditions are found and the fibers were subsequently applied for selective DNA analysis. Several experimental parameters such as stir-rate, desorption time, and buffer-type are optimized. The developed method was able to selectively extract the target DNA sequence (260 bp) in the presence of 100-fold excess interfering salmon testes DNA.

Supplementary files

Article information

DOI: https://doi.org/10.1039/D0AY00980F
Article type: Communication
Submitted: 15 May 2020
Accepted: 09 Jun 2020
First published: 09 Jun 2020
This article is Open Access

Download Citation

Anal. Methods, 2020,12, 3200-3204

Permissions

Request permissions

Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction

M. Varona, O. I. Shimelis and J. L. Anderson, Anal. Methods, 2020, 12, 3200 DOI: 10.1039/D0AY00980F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Analytical Methods

Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction

Social activity

Search articles by author

Spotlight

Advertisements