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Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases

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Abstract

RNA silencing and RNA decay are functionally interlaced, regulate gene expression and play a pivotal role in antiviral responses. As a counter-defensive strategy, many plant and mammalian viruses encode suppressors which interfere with both mechanisms. However, the protein interactions that connect these pathways remain elusive. Previous work reported that RNA silencing suppressors from different potyviruses, together with translation initiation factors EIF(iso)4E, interacted with the C-terminal region of the tobacco exoribonuclease RRP6-like 2, a component of the RNA decay exosome complex. Here, we investigate whether other viral silencing suppressors and cellular proteins might also bind RRP6-like exoribonucleases. A candidate search approach based on yeast two-hybrid protein interaction assays showed that three other unrelated viral suppressors, two from plant viruses and one from a mammalian virus, bound the C-terminus of the tobacco RRP6-like 2, the full-length of the Arabidopsis RRP6L1 protein and its C-terminal region. In addition, RRP6-like proteins were found to interact with members of the cellular double-stranded RNA-binding protein (DRB) family involved in RNA silencing. The C-terminal regions of RRP6L proteins are engaged in homotypic and heterotypic interactions and were predicted to be disordered. Collectively, these results suggest a protein interaction network that connects components of RNA decay and RNA silencing that is targeted by viral silencing suppressors.

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Acknowledgements

We thank Taline Elmayan, Dominique Gagliardi, Yuke He, Stuart MacFarlane, Xuemei Chen, Yuqin Cheng, Marie-Aude Tschopp, Valerian Dolja, Jianping Chen, Hernán Garcia-Ruiz and Amelia Nieto for providing materials; Shirley Burgess for correction of the text; Universidad Nacional de Córdoba and CONICET for facilities and support.

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The study was funded by CONICET.

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  1. Instituto Multidisciplinario de Biología Vegetal (IMBIV), CONICET, Universidad Nacional de Córdoba (UNC), Av. Vélez Sarsfield 299, CC 495, 5000, Córdoba, Argentina

    Miguel Ángel Freire

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Correspondence to Miguel Ángel Freire.

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Freire, M.Á. Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases. Virus Genes 56, 621–631 (2020). https://doi.org/10.1007/s11262-020-01775-z

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