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CRISPR-mediated knockdown of miR-214 modulates cell fate in response to anti-cancer drugs in HPV-negative and HPV-positive cervical cancer cells

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Abstract

Cervical cancer is the fourth most common cause of mortality in women worldwide. In this study we investigated the effect of a tumour suppressor microRNA miR-214 in modulating the cell death against chemotherapeutic drugs like Doxorubicin, Cisplatin and Paclitaxel. CRISPR-facilitated knockdown and plasmid-based overexpression of miR-214 was performed in cervical cancer cell lines HeLa, C33A and CaSki. It was observed that knocking out miR-214 resulted in reduced apoptosis and cell migration upon drug treatments; while overexpression of miR-214 resulted in marginal increase in apoptosis and cell migration when treated with drugs. However, miR-214 had very little effect on production of reactive oxygen species. Our results also indicate that Doxorubicin was least effective and Paclitaxel most effective in inducing cell death. A combination of miR-214 overexpression and Paclitaxel treatment was found to be most effective in inducing cell death in cervical cancer cells. Analysis of cell cycle phases followed by apoptotic markers also showed that miR-214 overexpression along with Paclitaxel treatment caused an increase in PARP and decline of PI-3 kinase/Akt levels. Therefore, miR-214 levels determine the fate of the cancer cell during chemotherapeutic treatment.

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Acknowledgements

This study was funded by DST-SERB, India.

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Authors and Affiliations

  1. Cancer Biology Lab, School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, 751 024, India

    Prakriti Sen, Sayam Ghosal, Rudranil Hazra, Rimjhim Mohanty, Solomon Arega & Niladri Ganguly

  2. Infection Biology Lab, School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, 751 024, India

    Bikash Sahu

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  1. Prakriti Sen
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  2. Sayam Ghosal
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  3. Rudranil Hazra
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  4. Rimjhim Mohanty
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  5. Solomon Arega
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  6. Bikash Sahu
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  7. Niladri Ganguly
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Correspondence to Niladri Ganguly.

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Communicated by Ullas Kolthur-Seetharam.

Corresponding editor: Ullas Kolthur-Seetharam

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Sen, P., Ghosal, S., Hazra, R. et al. CRISPR-mediated knockdown of miR-214 modulates cell fate in response to anti-cancer drugs in HPV-negative and HPV-positive cervical cancer cells. J Biosci 45, 80 (2020). https://doi.org/10.1007/s12038-020-00054-1

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