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A simple, economical, and high efficient protocol to produce in vitro miniature rose

  • Plant Tissue Culture
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Abstract

Due to its high commercial value, many studies on rose (Rosa hybrida L.) micropropagation have been published. However, there are a limited number of studies on rose in vitro flowering. These studies only focused on the roles of plant growth regulators in the formation and morphogenesis of flowers. In this protocol, cytokinin was confirmed to positively function in the induction of in vitro rose flowers. In fact, more than 40% of in vitro shoots were induced to flower when they were grown on a medium supplemented with benzylaminopurine (BA) (2 mg L−1) and IAA (0.1 mg L−1). In addition, this study showed that the growth medium supplemented with only coconut water (15 or 20% v/v) was very efficient to induce flowering of in vitro miniature rose plants (> 70%) after 60 d of subculture. In addition, the in vitro flowers were normal and almost similar to ex vitro flowers in terms of flower shape and color. Based on these results, a detailed procedure for in vitro miniature rose flower production is provided.

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Authors and Affiliations

  1. Faculty of Biotechnology, Ho Chi Minh City Open University, 97 Vo Van Tan Street, District 3, Ho Chi Minh City, Vietnam

    Nguyen Hoai Nguyen

  2. Department of Plant Biotechnology and Biotransformation, Faculty of Biology-Biotechnology, University of Science, Vietnam National University Ho Chi Minh City, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City, Vietnam

    Bui Van Le

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  1. Nguyen Hoai Nguyen
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  2. Bui Van Le
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Correspondence to Nguyen Hoai Nguyen.

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Editor: Jianxin Chen

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Nguyen, N.H., Van Le, B. A simple, economical, and high efficient protocol to produce in vitro miniature rose . In Vitro Cell.Dev.Biol.-Plant 56, 362–365 (2020). https://doi.org/10.1007/s11627-019-10043-1

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  • DOI: https://doi.org/10.1007/s11627-019-10043-1

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