Abstract
Wildfires are having both devastating and regenerative impacts on the ecosystems in the Pacific Northwest of North America. Balsamorhiza sagittata and B. deltoidea (balsamroot) are ecologically important species in this region, and B. sagittata populations are increasing, while B. deltoidea is critically imperiled. The aim of this research was to establish in vitro protocols for germination and regeneration of Balsamorhiza spp. to enable conservation efforts. It was hypothesized that karrikins, which are plant growth regulators released from burning plants during wildfires, would induce seed germination in Balsamorhiza spp. Three karrikins (KAR1, KAR2, and KAR11) were tested for the ability to enhance germination in these species at two levels (5 or 10 μM). KAR2 had the strongest positive effect on germination and induced 47% and 60% germination, respectively, in B. sagittata seeds compared to 14% germination obtained in the control (water agar media). In B. deltoidea, KAR2 treatment resulted in a germination rate of 73.1% and 100%, compared to 69% in the control. A germplasm collection of seedlings of both species was established for conservation and regeneration experiments. Thidiazuron treatment (10 μM) induced formation of embryo-like structures in seedlings of both B. sagittata and B. deltoidea, with regenerants originating from the crown of seedlings. The present study provides in vitro methods for conservation and mass propagation of Balsamorhiza species.
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References
Applestein C, Smith S (2014) Propagation protocol for production of propagules (seeds, cuttings, poles, etc.) Balsamorhiza deltoidea Nutt. seeds. US Department of Agriculture, Forest services, National Center for Reforestation, Nurseries, and Genetic Resources, Olympia
Banerjee A, Tripathi DK, Roychoudhury A (2019) The karrikin ‘calisthenics’: can compounds derived from smoke help in stress tolerance? Physiol Plant 165:290–302
Bujak CM, Dougher TAO (2017) Classification of seed dormancy and treatment of gibberellic acid to improve germination of arrowleaf balsamroot. Native Plant J18:32–41
Bürger M, Mashiguchi K, Lee HJ, Nakano M, Takemoto K, Seto Y, Yamaguchi S, Chory J (2019) Structural basis of karrikin and non-natural strigolactone perception in Physcomitrella patens. Plant Cell Rep 26:855–865
Chambers KJ, Bowen PA, Turner NJ, Keller PC (2006) Ethylene improves germination of arrow-leaved balsamroot seeds. Native Plants J 7:108–113
Chiwocha SDS, Dixon KW, Flematti GR, Ghisalberti E, Merritt D, Nelson D, Riseborough JM, Smith S, Stevens J (2009) Karrikins: a new family of plant growth regulators in smoke. Plant Sci 177:252–256
Committee on the Status of Endangered Wildlife in Canada (2009) COSEWIC assessment and update status report on the deltoid balsamroot Balsamorhiza deltoidea in Canada. Ottawa
Dellasala DA, Williams JE, Williams CD, Franklin JF (2004) Beyond smoke and mirrors: a synthesis of fire policy and science. Conserv Biol 18:976–986
Douglas GW, Ryan M (2001) Status of the deltoid balsamroot, Balsamorhiza deltoidea (Asteraceae) in Canada. Can Field-Nat 115:451–454
Drake D, Ewing K (1997) Germination requirements of 32 native Washington prairie species. In: Ewing PD and K (ed) Ecology and conservation of the South Puget Sound prairie landscape. The Nature Conservancy of Washington, Seattle, pp 181–191
Fazzino L, Kirkpatrick HE, Fimbel C (2011) Comparison of hand-pollinated and naturally-pollinated puget balsamroot (Balsamorhiza deltoidea Nutt.) to determine pollinator limitations on South Puget Sound lowland prairies. Northwest Sci 85:352–360
Filmon G (2004) Firestorm 2003- Provincial review. Government of the Province of British Columbia, Vancouver, British Columbia
Flematti GR, Dixon KW, Smith SM (2015) What are karrikins and how were they “discovered” by plants? BMC Biol 13:1–7
Flematti GR, Ghisalberti EL, Dixon KW, Trengove RD (2004) A compound from smoke that promotes seed germination. Science 305:977
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:150–158
Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10:8984–9000
Krock S, Smith S, Elliott C, Kennedy A, Hamman ST (2016) Using smoke-water and cold-moist stratification to improve germination of native prairie species. Native Plants J 17:19–2747
Link SO, Cruz RO, Harper BL, et al (2011) Shrub-steppe species germination trials and survival after outplanting on bare soils. USDA for Serv Proc RMRS-P-65 159–167
Meng Y, Shuai H, Luo X, Chen F, Zhou W, Yang W, Shu K (2017) Karrikins: regulators involved in Phytohormone signaling networks during seed germination and seedling development. Front Plant Sci 7:479–479
Monsen SB, Stevens R, Shaw NL (2004) Restoring western ranges and wildlands. Gen Tech Rep RMRS-GTR-136-vol-1 1:1–294
Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275
Nelson DC, Riseborough J-A, Flematti GR, Stevens J, Ghisalberti EL, Dixon KW, Smith SM (2009) Karrikins discovered in smoke trigger Arabidopsis seed germination by a mechanism requiring gibberellic acid synthesis and light. Plant Physiol 149:863–873
Nelson DC, Scaffidi A, Dun EA, Waters MT, Flematti GR, Dixon KW, Beveridge CA, Ghisalberti EL, Smith SM (2011) F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana. Proc Natl Acad Sci 108:8897–8902
Parks Canada Agency (2017) Multi-species action plan for Fort Rodd Hill National Historic Site of Canada. Ottawa
Reinhardt ED, Keane RE, Calkin DE, Cohen JD (2008) Objectives and considerations for wildland fuel treatment in forested ecosystems of the interior western United States. For Ecol Manag 256:1997–2006
Ruduś I, Cembrowska-Lech D, Jaworska A, Kępczyński J (2019) Involvement of ethylene biosynthesis and perception during germination of dormant Avena fatua L. caryopses induced by KAR1 or GA3. Planta 249:719–738
Sparg SG, Kulkarni MG, Light ME, Van Staden J (2005) Improving seedling vigour of indigenous medicinal plants with smoke. Bioresour Technol 96:1323–1330
Tilley D, St. John L, Shaw N (2012) Plant guide for arrowleaf balsamroot (Balsamorhiza sagittata). USDA-Natural Resources Conservation Service, Aberdeen Plant Materials Center. Aberdeen, Idaho.
Turner NJ (1997) Food plants of interior first peoples. Royal British Columbia Museum; 2nd Ed. Vancouver, British Columbia, Canada
Turner NJ (2014) Ancient pathways, ancestral knowledge: ethnobotany and ecological wisdom of indigenous peoples of northwestern North America. McGill-Queen’s University Press, Montreal & Kingston
Van Staden J, Jäger AK, Light ME, Burger BV (2004) Isolation of the major germination cue from plant-derived smoke. S Afr J Bot 70:654–659
Wang L, Waters MT, Smith SM (2018) Karrikin-KAI2 signalling provides Arabidopsis seeds with tolerance to abiotic stress and inhibits germination under conditions unfavourable to seedling establishment. New Phytol 219:605–618
Waters MT, Nelson DC, Scaffidi A, Flematti GR, Sun YK, Dixon KW, Smith SM (2012) Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis. Development 139:1285–1295
Wilkins BT (1957) Range use, food habits, and agricultural relationships of mule deer, Bridger Mountains, Montana. J Wildl Manag 21:159–169
Young JA, Evans RA (1978) Population dynamics after wildfires in sagebrush grasslands. J Range Manag 31:283–289
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Monthony, A.S., Baethke, K., Erland, L.A.E. et al. Tools for conservation of Balsamorhiza deltoidea and Balsamorhiza sagittata: Karrikin and thidiazuron-induced growth. In Vitro Cell.Dev.Biol.-Plant 56, 398–406 (2020). https://doi.org/10.1007/s11627-019-10052-0
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DOI: https://doi.org/10.1007/s11627-019-10052-0