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Mapping quantitative trait loci for resistance to watermelon bud necrosis orthotospovirus in watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai]

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Abstract

Watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] is an important cucurbit crop cultivated worldwide. In an effort to map WBNV resistance, the current study consisted of two populations derived from a WBNV resistant accession, IIHR-82 belonging to Citrullus lanatus var. citroides (Syn. Citrullus amarus). The first population is a backcross inbred population (BC1F6) of 141 families (Pop I) that was developed by crossing IIHR-82 to an elite cultivar Arka Manik. A resistant family, BIL-53 belonging to Pop I was crossed to IIHR-140 to develop F3 population, consisting of 112 families (Pop II). Each of these populations was evaluated for WBNV incidence for two years under natural epiphytotic conditions. A significant correlation was observed for various resistance traits across years and populations. The frequency distribution graphs of Area Under Disease Progress Curve for WBNV incidence in both the populations exhibited a continuous distribution, indicating that trait is quantitative in nature. A total of 163 markers were mapped for the Pop I which spread onto 15 linkage groups (LGs) spanning a total map length of 3310.17 cM with a mean marker interval of 20.31 cM. The linkage map for the Pop II was constructed with 135 markers spread over 12 linkage groups spanning a total length of 1965.53 cM with an average interval between markers of 14.56 cM.The QTL analysis for WBNV resistance related traits revealed 14 major QTL's for Pop I and 19 for Pop II with a maximum PVE upto 21.20%. Multi-trait QTL regions were observed on LG 3b for Pop I and LG 2, LG 4, LG 7 and LG 8 for Pop II. Importantly, we observed common QTL regions for plant survival on LG 2 and PDI on LG 3 and LG 8 for both the populations. As this is the first attempt to map QTL's for WBNV resistance, the results obtained in the present study may provide a guide for fine mapping multi-trait QTL regions.

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Acknowledgements

The authors would like to acknowledge Ministry of Social Justice and Empowerment, Government of India, for providing the Ph.D fellowship for first author and Department of Biotechnology, Government of India, for funding the program.

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  1. Indian Institute of Horticultural Research, Bengaluru, 560089, India

    G. C. Nagesh, R. N. Thontadarya, K. M. Swamy, R. Muttanna, D. C. Lakshmana Reddy, R. Asokan, M. Krishna Reddy & E. S. Rao

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  1. G. C. Nagesh
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  2. R. N. Thontadarya
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  3. K. M. Swamy
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Contributions

Conceived and designed the experiments: ESR; NGC, TRN, SKM and MR performed the experiments with assistance from MKR for phenotyping; LRDC for genotyping; AR for viral diagnostic assays; ESR and NGC wrote the paper. All the authors have discussed the results and commented on the manuscript.

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Correspondence to E. S. Rao.

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Nagesh, G.C., Thontadarya, R.N., Swamy, K.M. et al. Mapping quantitative trait loci for resistance to watermelon bud necrosis orthotospovirus in watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai]. Euphytica 216, 104 (2020). https://doi.org/10.1007/s10681-020-02632-8

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