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Endothelial Nitric Oxide Synthase Gene Polymorphisms (− 922A > G, − 786 T > C, Intron 4 b/a VNTR and 894 G > T) and Essential Hypertension: An Association Study with Haplotypes Analysis

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Abstract

Endothelial Nitric Oxide Synthase (eNOS) is an indispensable regulator of blood pressure through producing Nitric Oxide (NO). There is some evidence to suggest that eNOS gene polymorphisms are associated with Essential Hypertension (EHT). In this study, the potential association between eNOS 4a/4b, A922G, G894T, T786C gene polymorphisms and EHT as individual risk factors and as haplotypes are examined in the southern population of Iran (Bandar-Abbas). In this study, 200 EHT patients and 200 normotensive subjects which were matched for age and sex were included. Genotyping was performed by either utilizing Polymerase Chain Reaction (PCR) or PCR followed by Restriction Fragment length Polymorphism (RFLP) method. Our results demonstrated statistically significant associations between T786C, G894T, and 4a/4a and EHT (p < 0.05); however, A922G had no significant association with EHT (p > 0.05). Haplotype analysis also suggested that − 786C/− 922A/4a, − 786C/− 922A/4b and − 786C/− 922G/4a haplotypes were more frequent in EHT group than control group, hypothesizing a positive association with EHT. The present study has identified that the eNOS genetic variations are associated with EHT in southern population of Iran (Bandar-Abbas). These findings also suggested that a number of haplotypes of eNOS gene may be a driving factor for EHT susceptibility in respected population.

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Acknowledgments

Authors would like to express their sincerest appreciation to all subjects for participating in this study.

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Authors and Affiliations

  1. Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

    Zahra Farbood, Amirsaeed Sabeti Aghabozorgi, Azim Nejatizadeh, Leila Shams, Elahe Mansouri Babamansouri & Mohammad Shekari

  2. Hormozgan Cardiovascular Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

    Hossein Farshidi

  3. Centre for Heart Lung Innovation, St. Paul’s Hospital, Vancouver, BC, Canada

    Amirhossein Bahreyni

  4. Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

    Amirhossein Bahreyni

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  1. Zahra Farbood
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  2. Amirsaeed Sabeti Aghabozorgi
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  3. Azim Nejatizadeh
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  4. Hossein Farshidi
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  5. Leila Shams
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  7. Elahe Mansouri Babamansouri
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Correspondence to Mohammad Shekari.

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Farbood, Z., Sabeti Aghabozorgi, A., Nejatizadeh, A. et al. Endothelial Nitric Oxide Synthase Gene Polymorphisms (− 922A > G, − 786 T > C, Intron 4 b/a VNTR and 894 G > T) and Essential Hypertension: An Association Study with Haplotypes Analysis. Biochem Genet 58, 518–532 (2020). https://doi.org/10.1007/s10528-020-09953-2

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