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Genome sequence analysis of a novel Enterobacter cloacae phage, Ec_L1, belonging to the genus Eclunavirus

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Abstract

Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24% G+C content. Nineteen of the 85 putative proteins encoded by this phage have known functions, and no rRNA or tRNA genes were found. Comparative analysis of genome sequences suggested that phage Ec_L1 should be considered a member of the subfamily Tunavirinae, which includes T1-like phages. According to the International Committee on Taxonomy of Viruses (ICTV), phage Ec_L1 is the type member of the new genus “Eclunavirus”, whose name was derived from Ec_L1.

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Acknowledgements

The authors would like to thank the members of the research group of Professor Yigang Tong, Beijing University of Chemical Technology, for help with sequence analysis.

Funding

This work was supported by a China Postdoctoral Science Foundation Grant (2019M661105).

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Author notes

  1. Hongyu Ren and Zhen Li contributed equally.

Authors and Affiliations

  1. School of Bioengineering, Dalian University of Technology, Dalian, 116024, People’s Republic of China

    Hongyu Ren, Xiaoyu Li, Lili Wang & Yongping Xu

  2. Postdoctoral Work Center in Dalian SEM Biological Engineering Co. Ltd, Dalian, 116620, People’s Republic of China

    Zhen Li & Yongping Xu

  3. School of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng, 224051, People’s Republic of China

    Zhen Li

Authors
  1. Hongyu Ren
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  2. Zhen Li
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  3. Xiaoyu Li
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  4. Lili Wang
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  5. Yongping Xu
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Contributions

Hongyu Ren and Zhen Li contributed equally to this article and share first authorship.

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Correspondence to Yongping Xu.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: T. K. Frey.

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Ren, H., Li, Z., Li, X. et al. Genome sequence analysis of a novel Enterobacter cloacae phage, Ec_L1, belonging to the genus Eclunavirus. Arch Virol 165, 1929–1932 (2020). https://doi.org/10.1007/s00705-020-04672-w

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  • DOI: https://doi.org/10.1007/s00705-020-04672-w

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