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Luteimonas granuli sp. nov., Isolated from Granules of the Wastewater Treatment Plant

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Abstract

A Gram-reaction negative, aerobic, non-motile, light yellow colored, and rod-shaped bacterium (designated Gr-4T) isolated from granules of a wastewater treatment plant, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gr-4T was observed to grew optimally at 30 ºC and at pH 7.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gr-4T belongs to the genus Luteimonas of the family Xanthomonadaceae and was most closely related to Luteimonas padinae CDR SL 15T (99.1%), Luteimonas terricola DSM 22344T (98.5%) and Luteimonas arsenica 26-35T (97.6). The genome comprises 2,917,404 bp with a G+C content of 70.5 mol%. The ANI value between strain Gr-4T and Luteimonas padinae CDR SL 15T was 87.3%. The DNA–DNA relatedness value between strain Gr-4T and Luteimonas padinae CDR SL 15T, Luteimonas terricola DSM 22344T was 36.4 ± 1.3% and 14.2 ± 1.7%, respectively. The predominant quinone was Q-8. The major fatty acids were iso-C15:0, iso-C16:0 and summed feature 9 (comprising iso-C17:1ω9c and/or C16:0 10-methyl) supported the affiliation of strain Gr-4T to the genus Luteimonas. Moreover, the physiological, biochemical results, and low level of ANI and DNA–DNA relatedness value allowed the phenotypic and genotypic differentiation of strains Gr-4T from other Luteimonas species with validly published names. The novel isolate therefore represents a novel species, for which the name Luteimonas granuli sp. nov. is proposed, with the type strain Gr-4T (=KACC 16614T = JCM 18203T).

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Acknowledgements

This research was supported by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR), by a grant from the Korea Research Institute of Bioscience & Biotechnology (KRIBB) Research Initiative Program and by Korea Research Fellowship of Korea (NRF) funded by the Ministry of Science and ICT (Project No. 2019H1D3A1A02070958). (KRF) Program through the National Research Foundation.

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Authors and Affiliations

  1. Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan Taek Im

  2. AceEMzyme Co., Ltd, Academic Industry Cooperation, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan Taek Im

  3. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea

    Jun Mo Yeon & Hanna Choi

  4. Department of Food & Nutrition, Seoil University, Seoul, Republic of Korea

    Jae Hag Lee

  5. Department of Food Science & Bio Technology, Shinansan University, Ansan, Korea

    Sang Yong Kim & Ji-Hyang Wee

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  1. Muhammad Zubair Siddiqi
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  2. Jun Mo Yeon
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  3. Hanna Choi
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  4. Jae Hag Lee
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  5. Sang Yong Kim
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  7. Wan Taek Im
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Contributions

Conceived and designed the experiments: MZS, WTI. Performed the experiments: MZS, WTI. Analyzed the data: SYK, JHY, WTI. Wrote the paper: MZS.

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Correspondence to Wan Taek Im.

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Siddiqi, M.Z., Yeon, J.M., Choi, H. et al. Luteimonas granuli sp. nov., Isolated from Granules of the Wastewater Treatment Plant. Curr Microbiol 77, 2002–2007 (2020). https://doi.org/10.1007/s00284-020-02066-4

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  • DOI: https://doi.org/10.1007/s00284-020-02066-4

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