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Hymenobacter lutimineralis sp. nov., belonging to the family Hymenobacteraceae, isolated from zeolite

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Abstract

A red-pigmented bacterial strain, designated KIGAM108T, within the family Hymenobacteraceae was isolated from zeolite in the Gampo-41 mine of the Gyeongju, Republic of Korea. This strain was a Gram-negative, strictly aerobic, non-spore forming, rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence of strain KIGAM108T found that it was related to the genus Hymenobacter, with similarities of 96.6, 96.4, 95.5, and 95.0% to H. fastidiosus VUG-A124T, H. algoricola VUG-A23aT, H. crusticola MIMBbqt21T, and H. daecheongensis DSM 21074T, respectively. Strain KIGAM108T grew in the presence of 0–0.5% (w/v) NaCl at 4–37 °C and pH 6.0–10.0. This isolate contained MK-7 as a respiratory quinone. The polar lipids of strain KIGAM108T were identified as phosphatidylethanolamine, two unidentified aminophospholipids, one unidentified phospholipid and five unidentified lipids. The major fatty acids profile showed summed feature 3 (C16:1ω6c and/or C16:1ω7c) (22.3%), anteiso-C15:0 (17.1%), C16:1ω5c (13.3%), and iso-C15:0 (11.0%). The genomic DNA G + C content was 60.0 mol%. Based on the polyphasic taxonomic data, strain KIGAM108T is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter lutimineralis sp. nov. is proposed. The type strain is KIGAM108T (=KCTC 72263T =JCM 33444T).

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Funding

This research was supported by Collaborative Research Program of the Korea Food Research Institute (KFRI) funded by the Korea Institute of Geoscience and Mineral Resources (KIGAM). This research was supported by the Basic Research Project (20-3213) of the Korea Institute of Geoscience and Mineral Resources (KIGAM) funded by the Ministry of Science and ICT. This research was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MIST) (NRF-2019R1A2C1006038).

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Authors and Affiliations

  1. Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea

    Eui-Sang Cho & Myung-Ji Seo

  2. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    So-Lim Park, Young-Do Nam & Seong-Il Lim

  3. Department of Nanomaterials Science and Engineering, University of Science and Technology, Daejeon, 34113, Republic of Korea

    Dae-Young Kim

  4. Bacterial Research Team, Nakdonggang National Institute of Biological Resources, Sangju, 37242, Republic of Korea

    Dong-Hyun Jung

  5. Advanced Geo-Materials R&D Department, Pohang Branch, Korea Institute of Geoscience and Mineral Resources, Pohang, 37559, Republic of Korea

    Jae-Hwan Kim

  6. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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  1. Eui-Sang Cho
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  2. So-Lim Park
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Contributions

E-S C performed the experiments and drafted the manuscript. S-L P and S-I L contributed to strains isolation and morphological analyses. Y-D N contributed to genome sequencing and analysis. D-H J contributed to in silico phenotypic and genomic analysis. D-Y K contributed to the biochemical characterisation. J-H K and M-J S designed all the experiments and supervised the manuscript.

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Correspondence to Jae-Hwan Kim or Myung-Ji Seo.

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Cho, ES., Park, SL., Nam, YD. et al. Hymenobacter lutimineralis sp. nov., belonging to the family Hymenobacteraceae, isolated from zeolite. Antonie van Leeuwenhoek 113, 947–957 (2020). https://doi.org/10.1007/s10482-020-01402-9

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