1887

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Volume 101, Issue 8

Rapid generation of rotavirus single-gene reassortants by means of eleven plasmid-only based reverse genetics Free

Abstract

Reassortment is an important mechanism in the evolution of group A rotaviruses (RVAs), yielding viruses with novel genetic and phenotypic traits. The classical methods for generating RVA reassortants with the desired genetic combinations are laborious and time-consuming because of the screening and selection processes required to isolate a desired reassortant. Taking advantage of a recently developed RVA reverse genetics system based on just 11 cloned cDNAs encoding the RVA genome (11 plasmid-only system), we prepared a panel of simian SA11-L2 virus-based single-gene reassortants, each containing 1 segment derived from human KU virus of the G1P[8] genotype. It was shown that there was no gene-specific restriction of the reassortment potential. In addition to these 11 single-gene reassortants, a triple-gene reassortant with KU-derived core-encoding VP1–3 gene segments with the SA11-L2 genetic background, which make up a virion composed of the KU-based core, and SA11-L2-based intermediate and outer layers, could also be prepared with the 11 plasmid-only system. Finally, for possible clinical application of this system, we generated a series of VP7 reassortants representing all the major human RVA G genotypes (G1–4, G9 and G12) efficiently. The preparation of each of these single-gene reassortants was achieved within just 2 weeks. Our results demonstrate that the 11 plasmid-only system allows the rapid and reliable generation of RVA single-gene reassortants, which will be useful for basic research and clinical applications.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 11 plasmid-only system , G genotypes , group A rotavirus , reassortment and reverse genetics
Funding
This study was supported by the:
  • Mochida Memorial Foundation for Medical and Pharmaceutical Research
    • Principle Award Recipient: Satoshi Komoto
  • Takeda Science Foundation
    • Principle Award Recipient: Satoshi Komoto
  • Japan Society for the Promotion of Science (Award 18H02784)
    • Principle Award Recipient: Tetsushi Yoshikawa
  • Japan Society for the Promotion of Science (Award 15K08505, 18K07150)
    • Principle Award Recipient: Satoshi Komoto
  • Japan Agency for Medical Research and Development (Award 18fk0108018h0403, 18fk0108034h1102)
    • Principle Award Recipient: Satoshi Komoto
© 2020 The Authors
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2020-06-03
2024-04-20
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Rapid generation of rotavirus single-gene reassortants by means of eleven plasmid-only based reverse genetics
J. Gen. Virol. 101, 806 (2020); https://doi.org/10.1099/jgv.0.001443
/content/journal/jgv/10.1099/jgv.0.001443
/content/journal/jgv/10.1099/jgv.0.001443
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