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Effects of Selenium on Arsenic-Induced Liver Lesions in Broilers

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Abstract

The aim of the present study was to investigate the abilities of selenium to counteract the toxic damage of arsenic (As). Two hundred 1-day-old healthy male broilers were randomly divided into five groups and fed the following diets: control group (0.1 mg/kg As + 0.2 mg/kg Se), As group (3 mg/kg As + 0.2 mg/kg Se), As + Se group I (3 mg/kg As + 5 mg/kg Se), As + Se group II (3 mg/kg As + 10 mg/kg Se), and As + Se group III (3 mg/kg As + 15 mg/kg Se), respectively. The relative weight of the liver, hepatic protein content, GSH-Px levels, SOD activities, NO contents, iNOS and tNOS activities, and increased malondialdehyde contents, ALT and AST activities, and the apoptotic hepatocytes were analyzed. Adding 3 mg/kg arsenic to the diet caused the growth and development of chicken liver to be blocked, resulting in decrease of protein contents in liver tissue, decrease of SOD and GSH-Px activities, increase of MDA contents, decrease of NO contents, decrease of iNOS and TNOs activities, increase of ALT and AST activities, increase of apoptosis rates of liver cells. Compared to the 3-mg/kg arsenic group, adding 5 mg/kg and 10 mg/kg selenium, respectively, could repair the liver growth retardation and steatosis caused by arsenic, increase the protein contents in liver tissue, increase the activities of SOD and GSH-Px, reduce the contents of MDA, increase the contents of NO, enhance the activities of iNOS and TNOs, reduce the activities of ALT and AST, and reduce the rates of apoptosis of liver cells, in which the best effects are to add 10 mg/kg selenium. While 15 mg/kg of sodium selenite may induce progression of As-induced hepatic lesions, the results indicated that 5 and 10 mg/kg of sodium selenite supplied in the diet, through mechanisms of oxidative stress and apoptosis regulation, may ameliorate As-induced hepatic lesions in a dose-dependent manner.

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Funding

The present work was supported by the Changjiang Scholars & Innovative Research Team of Ministry of Education of China Funds (Grant no. IRTO848) and the China scholarship council in 2019 (201906915019).

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Author notes

  1. Zhihua Ren and Huidan Deng contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Province Key Laboratory of Animal Disease & Human Health; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, 625014, China

    Zhihua Ren, Huidan Deng, Youtian Deng, Wenjiao Tang, Qiang Wu, Zhicai Zuo, Hengmin Cui, Yanchun Hu, Shumin Yu & Junliang Deng

  2. Leshan City, Shizhong District of Animal Husbandry Bureau, Leshan, 614000, China

    Wenjiao Tang & Qiang Wu

  3. Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang District, Chengdu, 611130, China

    Sheng-yu Xu

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  1. Zhihua Ren
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Ren, Z., Deng, H., Deng, Y. et al. Effects of Selenium on Arsenic-Induced Liver Lesions in Broilers. Biol Trace Elem Res 199, 1080–1089 (2021). https://doi.org/10.1007/s12011-020-02222-8

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